catalog number :
MBS609897
products full name :
TCR, alpha, beta (T Cell Receptor, T-cell Receptor beta-1 Chain C Region, TRBC1, T-cell Receptor alpha Chain C Region, TRAC, TCRA) (FITC)
products short name :
[TCR, alpha, beta]
products name syn :
[Anti -TCR, alpha, beta (T Cell Receptor, T-cell Receptor beta-1 Chain C Region, TRBC1, T-cell Receptor alpha Chain C Region, TRAC, TCRA) (FITC)]
other names :
[TCR, partial]
reactivity :
Cynomolgus monkey
specificity :
Recognizes rat TCR, alpha, beta. Detects a T cell specific molecule consisting of two disulphide-linked subunits of 40 and 46kD molecular weight, respectively, on 97% of peripheral rat T cells as defined by the OX-52 marker. 3H2918 is mitogenic for unseparated spleen cells and for purified T cells. In the rat thymus, mature medullary cells express the 3H2918 determinant at the same level as peripheral T cells, whereas 94% of CD4- CD8- thymocytes are 3H2918 negative. It is most likely directed at a constant determinant of the rat alpha/beta heterodimeric T cell antigen receptor.
purity :
Purified by Ion Exchange chromatography.
form :
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% Sodium Azide. Labeled with FITC,
storage stability :
Store product at 4°C if to be used immediately within 2 weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
tested application :
Flow Cytometry (FC/FACS). Other applications not tested.
app notes :
Flow Cytometry: Neat-1:5. Use 10ul to label 10e6 lymphocytes in 100ul. Optimal dilutions to be determined by the researcher.
other info1 :
Grade: Highly Purified. Immunogen: Rat T Blasts and Erythrocytes. Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.
products categories :
Antibodies; Abs to Receptors
products description :
The T Cell Receptor alpha/beta (TCR) is expressed on T lymphocytes of all mouse strains tested. Plate-bound or soluble antibody activates a/b TCR-bearing T cells. In vitro and in vivo, the antibody can induce immature thymocytes to undergo apoptosis. In vivo administration of this antibody has been shown to deplete a/b TCR-bearing cells to near completion and prevent graft rejection.
ncbi acc num :
AAA61095.1