Actin, alpha, Smooth Muscle | MyBioSource MBS609618 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Actin, alpha, Smooth Muscle

catalog :

MBS609618

quantity :

5 mL

price :

410 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5F160

reactivity :

human, mouse, rat, chicken, cow

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS609618

products type :

Antibody

products full name :

Actin, alpha, Smooth Muscle

products short name :

Actin, alpha, Smooth Muscle

products name syn :

Anti -Actin, alpha, Smooth Muscle

other names :

actin, alpha 2, smooth muscle, aorta, isoform CRA_a; Actin, aortic smooth muscle; actin, aortic smooth muscle; alpha-cardiac actin; cell growth-inhibiting gene 46 protein; actin, alpha 2, smooth muscle, aorta; Alpha-actin-2; Cell growth-inhibiting gene 46 protein

other gene names :

ACTA2; ACTA2; AAT6; ACTSA; MYMY5; ACTSA; ACTVS

uniprot entry name :

ACTA_HUMAN

clonality :

Monoclonal

isotype :

IgG2a

clone :

5F160

host :

Mouse

reactivity :

Bovine, Chicken, Equine, Human, Mouse, Rat

specificity :

Recognizes alpha-smooth-muscle isoform of actin (Mr 43,000). Reactivities on cultured stress fibers of smooth muscle-derived cells. Tumors specificallly detected

purity :

Affinity Purified. Purified by Protein A affinity chromatography.

form :

Supplied as a liquid in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide.

storage stability :

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry (frozen and paraffin-embedded tissue as well as cytological material). Dilution: Immunohistochemistry: Ready-to-use on frozen sections. Paraffin embedded sections require protease pretreatment prior to antibody application. Incubate 1 hour at RT; extended with paraffin. Optimal dilution to be determined by researcher.

other info1 :

Immunogen: Synthetic N-terminus decapeptide of alpha-smooth-muscle isoform of actin.

products categories :

Antibodies; Abs to Actin Related Proteins

products description :

Represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation, cultured stress fibers of smooth muscle-derived cells, and some smooth muscle subtype fibroblasts.

ncbi gi num :

119570540

ncbi acc num :

EAW50155.1

uniprot acc num :

P62736

ncbi mol weight :

42,009 Da

ncbi pathways :

Muscle Contraction Pathway (106261); Myometrial Relaxation And Contraction Pathways (198759); Smooth Muscle Contraction Pathway (161025); Striated Muscle Contraction Pathway (198903); Vascular Smooth Muscle Contraction Pathway (96530); Vascular Smooth Muscle Contraction Pathway (96866)

ncbi summary :

The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]

uniprot summary :

ACTA2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the actin family. Protein type: Contractile; Cytoskeletal; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 10q23.3. Cellular Component: extracellular space; protein complex; smooth muscle contractile fiber; cytoplasm; cytosol; actin cytoskeleton. Molecular Function: protein kinase binding; ATP binding. Biological Process: muscle contraction; regulation of blood pressure; response to virus; vascular smooth muscle contraction. Disease: Multisystemic Smooth Muscle Dysfunction Syndrome; Moyamoya Disease 5; Aortic Aneurysm, Familial Thoracic 6

size1 :

5 mL

price1 :

410 USD

more info or order :

MyBioSource product webpage