Indian Hedgehog (IHH, Indian Hedgehog Protein, HHG-2) | MyBioSource MBS609380 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Indian Hedgehog (IHH, Indian Hedgehog Protein, HHG-2)

catalog :

MBS609380

quantity :

0.5 mg

price :

810 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

[11C22]

reactivity :

human, mouse

application :

western blot, ELISA

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS609380

products type :

Antibody

products full name :

Indian Hedgehog (IHH, Indian Hedgehog Protein, HHG-2)

products short name :

[Indian Hedgehog]

products name syn :

[Anti -Indian Hedgehog (IHH, Indian Hedgehog Protein, HHG-2)]

other names :

[indian hedgehog protein; Indian hedgehog protein; indian hedgehog protein; HHG-2; OTTHUMP00000206322; Indian hedgehog homolog; Indian hedgehog; HHG-2]

other gene names :

[IHH; IHH; BDA1; HHG2]

uniprot entry name :

IHH_HUMAN

clonality :

Monoclonal

isotype :

IgG2b

clone :

[11C22]

host :

Rat

reactivity :

Human, Mouse

specificity :

Recognizes the Nterminal peptide of mouse Indian Hedgehog (aa66240). No crossreactivity with rmDhh (aa23198 or aa199396), rmIhh (aa241449), rhShh (aa24197), or rmShh (aa25198 or aa199437).

purity :

Purified by Protein G affinity chromatography. Endotoxin: <0.1EU/ug (LAL)

form :

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.

concentration :

0.5 mg/mL (after reconstitution)

storage stability :

Lyophilized and reconstituted products are stable for 12 months after receipt at 20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at 20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

tested application :

Suitable for use in ELISA and Western Blot.Other applications not tested.

app notes :

ELISA (Direct): 0.51ug/ml. The detection range is ~10ng/well. Western Blot: 12ug/ml. The detection range os ~5ng/lane under nonreducing and reducing conditions. Optimal dilutions to be determined by the researcher.

other info1 :

Immunogen: Recombinant protein corresponding to Cys28Gly202 at the Nterminal of mouse Indian Hedgehog, expressed in E. coli(AAB49692; P97812).

other info2 :

Type: Mab

products categories :

Antibodies; Abs to Transcription Factors

products description :

The hedgehog (hh) gene encoding a secreted protein was originally identified in Drosophila as a segment polarity gene. The vertebrate homologues of Hh comprise several proteins including sonic hedgehog (Shh), Indian hedgehog (Ihh), and Desert hedgehog (Dhh). Hedgehog proteins are important signaling molecules during embryonic development and are highly conserved within and across species. Mouse and human Ihh share 100% aa identity in the signaling domain, while mouse Ihh and Shh share 90% aa identity in the N terminal signaling domain. Ihh mRNA expression is detected in fetal lung, gut, stomach, liver, kidney, pancreas and strongly in cartilage, in growth regions of the developing bone. Ihh, along with parathyroid hormone related protein, regulate the rate of chondrocyte proliferation and differentiation. Ihh is also involved in yolk sac vasculogenesis, playing an important role in differentiation of epiblast cells into endothelial and red blood cells. Mouse Ihh cDNA encodes a 41aa polypeptide with a predicted 27aa signal peptide. This polypeptide is cleaved to generate a 45kD precursor protein that undergoes the same post translation processing as Shh. An autocatalytic reaction yields a 19kD amino terminal domain Ihh-N protein that retains all known signaling capabilities, and a 23kD carboxy terminal domain Ihh-C protein. Since hydrophobic modifications to Shh, including the substitution of the N terminal cysteine residue with two hydrophobic isoleucine residues, can also increase its potency, a similar modification was made for Ihh. This modified form also shows increased potency in a bioassay measuring induction of alkaline phosphatase. At the cell surface, Hedgehog activity is mediated by a multicomponent receptor complex involving the 12 pass transmembrane protein Patched (Ptc) which binds Hedgehogs with high affinity and Smoothened (Smo), a signaling seven transmembrane G protein coupled receptor.

ncbi gi num :

119392086

ncbi acc num :

AAB49692

ncbi gb acc num :

NM_002181.3

uniprot acc num :

P97812

ncbi mol weight :

45,251 Da

ncbi pathways :

Class B/2 (Secretin Family Receptors) Pathway (106378); Endochondral Ossification Pathway (198812); GPCR Ligand Binding Pathway (161020); Hedgehog Signaling Pathway (198835); Hedgehog Signaling Pathway (83063); Hedgehog Signaling Pathway (474); Signaling By GPCR Pathway (106356); Signaling Events Mediated By The Hedgehog Family Pathway (137950)

ncbi summary :

This gene encodes a member of the hedgehog family of secreted signaling molecules. Hedgehog proteins are essential regulators of a variety of developmental processes including growth, patterning and morphogenesis. The encoded protein specifically plays a role in bone growth an differentiation. Mutations in this gene are the cause of brachydactyly type A1 which is characterized by shortening or malformation of the phalanges. Mutations in this gene are also the cause of acrocapitofemoral dysplasia. [provided by RefSeq]

uniprot summary :

IHH: Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP). Expressed in embryonic lung, and in adult kidney and liver. Belongs to the hedgehog family. Chromosomal Location of Human Ortholog: 2q33-q35. Cellular Component: extracellular matrix; extracellular space; plasma membrane. Molecular Function: peptidase activity; patched binding; calcium ion binding. Biological Process: vitelline membrane formation; proteoglycan metabolic process; maternal process involved in pregnancy; positive regulation of collagen biosynthetic process; cell maturation; cell fate specification; embryonic pattern specification; proteolysis; response to estradiol stimulus; positive regulation of T cell differentiation in the thymus; smooth muscle development; cell-cell signaling; embryonic digestive tract morphogenesis; regulation of growth; positive regulation of mesenchymal cell proliferation; pancreas development; positive regulation of smoothened signaling pathway; heart looping; skeletal development; bone resorption; negative regulation of immature T cell proliferation in the thymus; smoothened signaling pathway; intein-mediated protein splicing; in utero embryonic development; multicellular organism growth; negative regulation of eye pigmentation; negative regulation of T cell differentiation in the thymus; negative regulation of signal transduction; patterning of blood vessels; osteoblast differentiation; positive regulation of chondrocyte differentiation; cartilage development; neuron development; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; embryonic camera-type eye morphogenesis; positive regulation of epithelial cell proliferation; positive regulation of alpha-beta T cell differentiation; negative regulation of apoptosis; negative regulation of alpha-beta T cell differentiation. Disease: Brachydactyly, Type A1; Acrocapitofemoral Dysplasia

size1 :

0.5 mg

price1 :

810 USD

more info or order :

MyBioSource product webpage