antibody

GLUT2 (Solute Carrier Family 2, Facilitated Glucose Transporter Member 2, Glucose Transporter Type 2, Liver, GLUT-2, SLC2A2)

MBS609124

0.1 mg

640 USD

monoclonal

rat

nonconjugated

11C102

western blot, flow cytometry, FACS

Antibody

GLUT2 (Solute Carrier Family 2, Facilitated Glucose Transporter Member 2, Glucose Transporter Type 2, Liver, GLUT-2, SLC2A2)

GLUT2

Anti -GLUT2 (Solute Carrier Family 2, Facilitated Glucose Transporter Member 2, Glucose Transporter Type 2, Liver, GLUT-2, SLC2A2)

GLUT2; Solute carrier family 2, facilitated glucose transporter member 2; solute carrier family 2, facilitated glucose transporter member 2; liver-type glucose transporter; glucose transporter type 2, liver; solute carrier family 2 (facilitated glucose transporter), member 2; Glucose transporter type 2, liver

Slc2a2; Slc2a2; Glut2; Glut-2; AI266973; Glut-2; Glut2

GTR2_MOUSE

Monoclonal

IgG2b

11C102

Rat

Mouse

Recognizes mouse GLUT2.

Affinity Purified. Purified by Protein A/G affinity chromatography

Supplied as a lyophilized powder from PBS. Reconstitute with 100ul of sterile PBS.

~1mg/ml(after reconstitution)

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB), Flow Cytometry (FC/FACS)

Suitable for use in Western Blot and Flow Cytometry. Dilution: Western Blot: 1-5ug/ml (ECL). Do not to heat the samples prior to loading to prevent gluts aggregation. Mouse tissues or mouse insulinoma beta TC-6 cells can be used as positive control. Flow Cytometry: ~10ug/ml (10e5 cells) Perform the antibody binding in small volume of ~200ul.

Immunogen: Recombinant corresponding to mouse GLUT2. Epitope: Extracellular domain

Antibodies; Abs to Glucose Transporters

Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1- Glut-14, Mol. Wt. 40-80kD) and Sodium glucose co-transporter- 1 (SGLT-1, 662aa; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and c-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells. Glut-2 provides glucose to the liver and pancreatic cells. Glut-3 is the main transporter in neurons, whereas Glut-4 is primarily expressed in muscle and adipose tissue and regulated by insulin. Glut-5 transports fructose in intestine and testis.

683589

P14246

57,107 Da

Carbohydrate Digestion And Absorption Pathway (170722); Carbohydrate Digestion And Absorption Pathway (170654); Facilitative Na+-independent Glucose Transporters Pathway (366423); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (366452); Glucose Transport Pathway (366420); Glycolysis And Gluconeogenesis Pathway (198376); Hexose Transport Pathway (366419); Maturity Onset Diabetes Of The Young Pathway (83293); Maturity Onset Diabetes Of The Young Pathway (508); Metabolism Of Carbohydrates Pathway (366891)

GLUT2: an integral membrane facilitative glucose transporter. One of 13 members of the human equilibrative glucose transport protein family. Present at high levels in the plasma membrane of pancreatic b-cells where it initiates the first step in glucose-stimulated insulin secretion, a glucose metabolism-dependent signaling mechanism. May mediate the bidirectional transfer of glucose across the plasma membrane of hepatocytes. May also participate with the Na( +2)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney. Required for glucose sensitivity in the hypothalamus and brain stem. Involved in the control of food intake and stimulation of glucose uptake by peripheral tissues. Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter, SLC family; Transporter. Cellular Component: membrane; basolateral plasma membrane; brush border membrane; cytoplasm; plasma membrane; integral to membrane; cytosol; brush border; endosome. Molecular Function: D-glucose transmembrane transporter activity; dehydroascorbic acid transporter activity; transporter activity; glucose transmembrane transporter activity; transmembrane transporter activity; substrate-specific transmembrane transporter activity; insulin receptor binding. Biological Process: carbohydrate utilization; transport; carbohydrate transport; glucose transport; transmembrane transport

0.1 mg

640 USD