Troponin I, cardiac muscle, PHOSPHORYLATED (Cardiac Troponin I, TNNI3, TNNC1) | MyBioSource MBS609006 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Troponin I, cardiac muscle, PHOSPHORYLATED (Cardiac Troponin I, TNNI3, TNNC1)

catalog :

MBS609006

quantity :

0.2 mg

price :

525 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

10F563

reactivity :

human, rat, dog, cow

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS609006

products type :

Antibody

products full name :

Troponin I, cardiac muscle, PHOSPHORYLATED (Cardiac Troponin I, TNNI3, TNNC1)

products short name :

Troponin I, cardiac muscle, PHOSPHORYLATED

products name syn :

Anti -Troponin I, cardiac muscle, PHOSPHORYLATED (Cardiac Troponin I, TNNI3, TNNC1)

other names :

troponin I, cardiac muscle; Troponin I, cardiac muscle; troponin I, cardiac muscle; troponin I type 3 (cardiac); Cardiac troponin I

other gene names :

TNNI3; TNNI3; CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF; MGC116817; TNNC1

uniprot entry name :

TNNI3_HUMAN

clonality :

Monoclonal

isotype :

IgG2b

clone :

10F563

host :

Mouse

reactivity :

Bovine, Canine, Feline, Goat, Human, Porcine, Rat

specificity :

Recognizes the phosphorylated form of human cardiac Troponin I (cTnI). Recognizes free cTnI and cTnI in complex with other Troponin components. It does not cross-react with skeletal Troponin I. Species Crossreactivity: rat, feline, bovine, canine, goat and porcine.

purity :

Affinity Purified. Purified by Protein A affinity chromatography.

form :

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

storage stability :

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

tested application :

ELISA (EL/EIA), Western Blot (WB)

app notes :

Suitable for use in ELISA and Western Blot. Dilution: Western Blot: For the detection of phosphoproteins, phosphatase inhibitors such as 10mM sodium fluoride and 1mM sodium orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

other info1 :

Immunogen: Phosphorylated form of human cardiac Troponin I.

other info2 :

Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice.

products categories :

Antibodies; Abs to Cardiac Markers

products description :

Troponin I is a 24kD subunit of Troponin, a protein found on muscle tissue responsible for muscle contraction. When calcium binds to Troponin C, a conformational change moves Troponin I and tropomyosin out of the way of the myosin binding sites on the actin filaments in muscles. This allows cross-bridging to occur and the muscle to contract. Levels of cTnI in serum are commonly used as a marker for cardiac cell damage and death. Phosphorylation of cTnI results in changes in the activity and function of Troponin. One possible form of phosphorylation is by Protein kinase A. Protein kinase A phosphorylation of serine 22 and serine 23 reduces calcium sensitivity of Troponin and leads to an acceleration in relaxation. Changes in cTnI phosphorylation have been reported in failing hearts.

ncbi gi num :

151101270

ncbi acc num :

NP_000354.4

ncbi gb acc num :

NM_000363.4

uniprot acc num :

P19429

ncbi mol weight :

24,008 Da

ncbi pathways :

Cardiac Muscle Contraction Pathway (93344); Cardiac Muscle Contraction Pathway (93992); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Hypertrophic Cardiomyopathy (HCM) Pathway (114229); Hypertrophic Cardiomyopathy (HCM) Pathway (106591); Muscle Contraction Pathway (106261); Striated Muscle Contraction Pathway (106262); Striated Muscle Contraction Pathway (198903)

ncbi summary :

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq]

uniprot summary :

TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family. Protein type: Motility/polarity/chemotaxis; Actin-binding; Motor. Chromosomal Location of Human Ortholog: 19q13.4. Cellular Component: sarcomere; troponin complex; cytosol. Molecular Function: troponin T binding; troponin C binding; protein domain specific binding; protein binding; metal ion binding; calcium channel inhibitor activity; actin binding; calcium-dependent protein binding; protein kinase binding. Biological Process: cellular calcium ion homeostasis; regulation of smooth muscle contraction; heart contraction; regulation of systemic arterial blood pressure by ischemic conditions; heart development; ventricular cardiac muscle morphogenesis; vasculogenesis; negative regulation of ATPase activity; cardiac muscle contraction; muscle filament sliding. Disease: Cardiomyopathy, Dilated, 1ff; Cardiomyopathy, Familial Restrictive, 1; Cardiomyopathy, Familial Hypertrophic, 7; Cardiomyopathy, Dilated, 2a

size1 :

0.2 mg

price1 :

525 USD

more info or order :

MyBioSource product webpage