Fibrillarin (FBL, 34kD Nucleolar Scleroderma Antigen, rRNA 2'-O-methyltransferase fibrillarin, FLRN, FIB1, Nop1p, RNU3IP1) | MyBioSource MBS608427 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Fibrillarin (FBL, 34kD Nucleolar Scleroderma Antigen, rRNA 2'-O-methyltransferase fibrillarin, FLRN, FIB1, Nop1p, RNU3IP1)

catalog :

MBS608427

quantity :

0.1 mg

price :

605 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[8C147]

reactivity :

human

application :

western blot, other

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS608427

products type :

Antibody

products full name :

Fibrillarin (FBL, 34kD Nucleolar Scleroderma Antigen, rRNA 2'-O-methyltransferase fibrillarin, FLRN, FIB1, Nop1p, RNU3IP1)

products short name :

[Fibrillarin]

products name syn :

[Anti -Fibrillarin (FBL, 34kD Nucleolar Scleroderma Antigen, rRNA 2'-O-methyltransferase fibrillarin, FLRN, FIB1, Nop1p, RNU3IP1)]

other names :

[fibrillarin; rRNA 2'-O-methyltransferase fibrillarin; rRNA 2'-O-methyltransferase fibrillarin; 34-kD nucleolar scleroderma antigen; 34 kDa nucleolar scleroderma antigen; RNA, U3 small nucleolar interacting protein 1; fibrillarin; 34 kDa nucleolar scleroderma antigen]

other gene names :

[FBL; FBL; FIB; FLRN; RNU3IP1; FIB1; FLRN]

uniprot entry name :

FBRL_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

[8C147]

host :

Mouse

specificity :

Recognizes human Fibrillarin.

purity :

Affinity Purified. Purified by Protein G affinity chromatography.

form :

Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.

concentration :

0.1 mg/mL

storage stability :

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior removing the cap.

tested application :

Western Blot (WB). Other applications not tested.

app notes :

Optimal dilutions to be determined by the researcher.

image1 heading :

Testing Data

other info1 :

Immunogen: Partial sequence of recombinant full-length protein to human Fibrillarin

products categories :

Antibodies; Abs to Enzymes, Methyltransferase

products description :

Nop1p / Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is fibrillarin (accession P22087) a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (Mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal 80 amino acids contain multiple copies based on the peptide RGG, and the remaining 240 amino acids consist of the fibrillarin domain. A fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologous to the other sequences throughout all of the fibrillarin domain. The structure of this molecule has been determined and shown to consist of 2 extended beta-sheets flanked by 4 alpha-helixes. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a 34kDa protein which was subsequently found to be fibrillarin. Fibrillarin is an excellent marker for the nucleolus.

ncbi gi num :

31395

ncbi acc num :

NM_001436

uniprot acc num :

P22087

ncbi pathways :

Ribosome Biogenesis In Eukaryotes Pathway (199378); Ribosome Biogenesis In Eukaryotes Pathway (199266); TNF-alpha/NF-kB Signaling Pathway (198884)

ncbi summary :

This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq]

uniprot summary :

FBL: Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2 - hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Interacts with NOLC1. Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles that contain NHP2L1, FBL, NOP5 and NOP56, plus a guide RNA. It is associated with the U3, U8, U13, X and Y small nuclear RNAs. Component of several ribosomal and nucleolar protein complexes. Interacts with PRMT5 and UTP20. Interacts with DDX5. Belongs to the methyltransferase superfamily. Fibrillarin family. Protein type: EC 2.1.1.-; RNA-binding; Nucleolus; Methyltransferase; RNA processing. Chromosomal Location of Human Ortholog: 19q13.1. Cellular Component: Cajal body; granular component; membrane; nucleolus; nucleus. Molecular Function: protein binding; RNA binding. Biological Process: osteoblast differentiation; snoRNA metabolic process; tRNA processing; rRNA processing

size1 :

0.1 mg

price1 :

605 USD

more info or order :

MyBioSource product webpage