antibody

Neural Cell Adhesion Molecule L1 (L1CAM, L1 cell adhesion molecule, CAML1, CD171, HSAS, HSAS1, MASA, MIC5, N-CAML1, N-CAM L1, S10, SPG1) (PE)

MBS608049

100 Tests

600 USD

monoclonal

rat

PE

9H148

human, mouse

flow cytometry, FACS

Antibody

Neural Cell Adhesion Molecule L1 (L1CAM, L1 cell adhesion molecule, CAML1, CD171, HSAS, HSAS1, MASA, MIC5, N-CAML1, N-CAM L1, S10, SPG1) (PE)

Neural Cell Adhesion Molecule L1

Anti -Neural Cell Adhesion Molecule L1 (L1CAM, L1 cell adhesion molecule, CAML1, CD171, HSAS, HSAS1, MASA, MIC5, N-CAML1, N-CAM L1, S10, SPG1) (PE)

neural cell adhesion molecule L1 isoform 2; Neural cell adhesion molecule L1; neural cell adhesion molecule L1; OTTHUMP00000025992; OTTHUMP00000025993; OTTHUMP00000063091; antigen identified by monoclonal antibody R1; L1 cell adhesion molecule

L1CAM; S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1

L1CAM_HUMAN

Monoclonal

IgG2a

9H148

Rat

Mouse

Recognizes the mouse Neural Cell Adhesion Molecule L1.

Affinity Purified. Purified by Protein G affinity chromatography.

Supplied as a liquid in saline, 0.5% BSA, 0.1% sodium azide. Labeled with R-Phycoerythrin (PE).

0.025 mg/ml

Store product at 4 degree C in the dark. DO NOT FREEZE. Stable at 4 degree C for 12 months after receipt, as n undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior ot removing the cap.

Flow Cytometry (FC/FACS)

Suitable for use in Flow Cytometry. Dilution: Flow Cytometry: 10ul labels 1-2.5x10e5 cells. Validated using mouse splenocytes. Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of mouse IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction. 2. After blocking, add 10ul of L0010-07 to 1-2.5x10e5 cells and incubate for 30 minutes at RT. 3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer (F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Mouse Lyse Buffer. 4. Resuspend the cells in Flow Cytometry Staining Buffer for analysis by flow cytometry. As a control for this analysis, cells in a separate tube should be treated with PE-labeled rat IgG2a antibody.

Immunogen: Mouse cerebellum-derived partially purified Neural Cell Adhesion Molecule L1.

Antibodies; Abs to Cancer Markers

The Neural Cell Adhesion Molecule L1 (NCAM-L1) is an Immunoglobulin superfamily (IgSF) adhesion molecule critical for neural development. NCAM-L1 binds homophilically and heterophilically with adhesion molecules including Axonin-1, CD9, Neurocan, and Integrins. Mutations of the gene encoding NCAM-L1 are associated with a subset of X-linked mental retardation syndromes, schizophrenia, and other neurological disorders. Cancer cells can proteolytically cleave NCAM-L1, releasing the soluble ectodomain. Within the ECD, human NCAM-L1 shares 86% and 87% aa identity with mouse and rat NCAM-L1, respectively.

13435353

NP_076493.1

NM_024003.2

P32004

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); Basigin Interactions Pathway (106065); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (106062); Hemostasis Pathway (106028); Interaction Between L1 And Ankyrins Pathway (161005); L1CAM Interactions Pathway (161003)

The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant. [provided by RefSeq]

NCAM-L1: cell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons. Two splice-variant isoforms have been described. Protein type: Membrane protein, integral; Cell adhesion. Chromosomal Location of Human Ortholog: Xq28. Cellular Component: presynaptic membrane; cell surface; focal adhesion; plasma membrane; integral to membrane; terminal button; external side of plasma membrane. Molecular Function: integrin binding; identical protein binding; protein self-association; sialic acid binding. Biological Process: nervous system development; axon guidance; positive regulation of calcium-mediated signaling; chemotaxis; heterophilic cell adhesion; leukocyte adhesion; cell surface receptor linked signal transduction; cell-cell adhesion mediated by integrin; homotypic cell-cell adhesion; positive regulation of cell-cell adhesion; blood coagulation; homophilic cell adhesion; cell adhesion; leukocyte migration. Disease: Hydrocephalus Due To Congenital Stenosis Of Aqueduct Of Sylvius; Corpus Callosum, Partial Agenesis Of, X-linked; Masa Syndrome

100 Tests

600 USD