product summary
request information :
company name :
MyBioSource
product type :
antibody
product name :
a-Actin, Smooth Muscle
catalog :
MBS607734
quantity :
0.05 ml
price :
575 USD
clonality :
monoclonal
host :
mouse
reactivity :
chicken, rat, human
application :
enzyme immunoassay, immunohistochemistry, western blot, ELISA
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS607734
products type :
Antibody
products full name :
a-Actin, Smooth Muscle
products short name :
a-Actin, Smooth Muscle
products name syn :
Anti -a-Actin, Smooth Muscle
other names :
actin, alpha skeletal muscle; Actin, alpha skeletal muscle; actin, alpha skeletal muscle; a-actin; alpha-actin-1; actin, alpha 1, skeletal muscle; Alpha-actin-1
other gene names :
ACTA1; ACTA1; ACTA
uniprot entry name :
ACTS_CHICK
clonality :
Monoclonal
isotype :
IgG2a
clone :
6G1
host :
Host: Mouse; Source: Human
reactivity :
Chicken, Human, Rat
specificity :
Reacts exclusively with alpha-smooth muscle actin which is typical for vascular and visceral
purity :
Purified. Purified
form :
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
storage stability :
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
tested application :
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
app notes :
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immuno-electron Microscopy. Dilution: Western Blot: 1:10,000-1:50,000. Immunohistochemistry (Paraffin): 1:10-1:100
other info1 :
Immunogen: Peptide comprising the first 10 amino acids of a-smooth muscle actin with an acetylated N-terminus coupled to keyhole limpet hemocyanin via the C-terminal cysteine (Ac-EEEDSTALVC).
other info2 :
Hybridoma: Derived by fusion of Sp 2/0 mouse myeloma cells with spleen cells from a BALB/c mouse.
products categories :
Antibodies; Abs to Actin Related Proteins
products description :
Among the six actin isoforms described in mammals, two are found in virtually all cells (beta- and gamma-cytoplasmic), two are detected in smooth muscle cells (alpha- and gamma-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (a-skeletal) and one in cardiac (a-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus and the sequences of each of them are perfectly conserved in higher vertebrates. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition, it has also been shown to appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases.
ncbi gi num :
71894831
ncbi acc num :
NP_001026234.1
ncbi gb acc num :
NM_001031063.1
uniprot acc num :
P68139
ncbi mol weight :
42,051 Da
uniprot summary :
Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Subunit structure: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Subcellular location: Cytoplasm cytoskeleton. Post-translational modification: Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization . By similarity. Miscellaneous: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. Sequence similarities: Belongs to the actin family.
size :
0.05 ml
price :
575 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!