catalog number :
MBS605904
products full name :
Hepatitis C Virus, Helicase, NS3 (HCV)
products short name :
Hepatitis C Virus, Helicase, NS3
products name syn :
Anti -Hepatitis C Virus, Helicase, NS3 (HCV)
specificity :
Recognizes Hepatitis C Virus, Helicase, NS3 (HCV). Reactive with genotypes 1a, 1b and 2a.
purity :
Affinity Purified. Purified by Protein A affinity chromatography.
form :
Supplied as a liquid in 0.01M PBS, pH 7.2, 0.1% sodium azide.
storage stability :
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
tested application :
ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
app notes :
Suitable for use in ELISA, Western Blot, and Immunofluorescence.
other info1 :
Immunogen: Recombinant Hepatitis C Virus, Helicase, NS3 (HCV)
products categories :
Antibodies; Abs to Infectious Disease Hepatitis
products description :
HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000aa. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70kD.