Activation-induced Cytidine Deaminase (AICDA, AID, ARP2, CDA2, Cytidine Aminohydrolase, HIGM2, Integrated into Burkitt's Lymphoma Cell Line Ramos) | MyBioSource MBS605724 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Activation-induced Cytidine Deaminase (AICDA, AID, ARP2, CDA2, Cytidine Aminohydrolase, HIGM2, Integrated into Burkitt's Lymphoma Cell Line Ramos)

catalog :

MBS605724

quantity :

0.1 mL

price :

550 USD

clonality :

monoclonal

host :

unidentified

conjugate :

nonconjugated

clone name :

[10g133]

reactivity :

human, mouse

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot analysis of extracts from P3HR-1 and Ramos cells using MBS605724.

image 2 :

Western Blot analysis of immunoprecipitates from HeLa cell extracts overexpressing myc-tagged AID. Expression of myc-AID was confirmed (lane 1). Immunoprecipitations using cell lysates with or without expression of myc-AID were performed using MBS605724 (anti-AID antibody), M9601-30 (anti-Myc-Tag antibody), mouse IgG, or rabbit IgG. Immunoblot was performed using M9601-30 (anti-Myc-Tag antibody).

product information

catalog number :

MBS605724

products type :

Antibody

products full name :

Activation-induced Cytidine Deaminase (AICDA, AID, ARP2, CDA2, Cytidine Aminohydrolase, HIGM2, Integrated into Burkitt's Lymphoma Cell Line Ramos)

products short name :

[Activation-induced Cytidine Deaminase]

products name syn :

[Anti -Activation-induced Cytidine Deaminase (AICDA, AID, ARP2, CDA2, Cytidine Aminohydrolase, HIGM2, Integrated into Burkitt's Lymphoma Cell Line Ramos)]

other names :

[activation-induced cytidine deaminase; AID; activation-induced cytidine deaminase; OTTHUMP00000239182; OTTHUMP00000239185; OTTHUMP00000239186; cytidine aminohydrolase; integrated into Burkitt's lymphoma cell line Ramos; activation-induced cytidine deaminase]

other gene names :

[AICDA; AID; AID; ARP2; CDA2; HIGM2; AICDA]

uniprot entry name :

Q546Y9_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

[10g133]

host :

unknown

reactivity :

Mouse

specificity :

Recognizes endogenous levels of human AID protein.

purity :

Ascites

form :

Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol.

storage stability :

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

tested application :

Western Blot (WB), Immunoprecipitation (IP). Other applications not tested.

app notes :

Western Blot: 1:1000. Immunoprecipitation: 1:50. Optimal dilutions to be determined by the researcher.

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

other info1 :

Immunogen: Synthetic peptide corresponding to residues of human AID.

products categories :

Antibodies; Abs to Enzymes

products description :

Activation-induced cytidine deaminase (AID) is thought to modify RNA due to its high homology to the RNA editing enzyme APOBEC-1. This function, however, has not been confirmed in in vitro studies, which show that AID has significant cytidine deaminase activity, and that this activity is blocked by zinc chelation. The B cell immune system must specifically recognize several infectious agents, which vastly outnumber immunoglobulin gene segments present in a given organism. Mechanisms such as somatic hypermutation, isotype switch recombination and gene conversion introduce diversity and specificity to the immune system. Analysis of mouse models and patients with AID deficiency has established a link between all three of these mechanisms and AID function. AID protein is detected in germinal center centroblast and germinal center derived lymphomas (Burkitt lymphoma), but not in pre-germinal center B cells or post-germinal center neoplasms (B cell chronic lymphocytic leukemia and multiple myeloma).

ncbi gi num :

10190700

ncbi acc num :

NP_065712.1

ncbi gb acc num :

NM_020661.2

uniprot acc num :

Q546Y9

ncbi pathways :

IL4-mediated Signaling Events Pathway (137933); Intestinal Immune Network For IgA Production Pathway (128760); Intestinal Immune Network For IgA Production Pathway (128670); Primary Immunodeficiency Pathway (83125); Primary Immunodeficiency Pathway (537)

ncbi summary :

This gene encodes a RNA-editing deaminase that is a member of the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. Defects in this gene are the cause of autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2). [provided by RefSeq]

size1 :

0.1 mL

price1 :

550 USD

more info or order :

MyBioSource product webpage