FOLR1 (Folate receptor 1 (adult) Adult folate-binding protein, FBP, Folate receptor, adult, Folate receptor 1, Folate receptor alpha, FOLR, FR-alpha, KB cells FBP, MOv18, Ovarian tumor-associated antigen MOv18) | MyBioSource MBS605669 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

FOLR1 (Folate receptor 1 (adult) Adult folate-binding protein, FBP, Folate receptor, adult, Folate receptor 1, Folate receptor alpha, FOLR, FR-alpha, KB cells FBP, MOv18, Ovarian tumor-associated antigen MOv18)

catalog :

MBS605669

quantity :

0.1 mg

price :

695 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[9H125]

reactivity :

human

application :

enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot detection of human FOLR1 using MBS605669. Lysates were prepared from Hela cells in non-reducing sample buffer, resolved by SDS-PAGE (30ug total protein/lane), and transferred to an Immobilon-P membrane. The blot was developed with MBS605669 (2ug/ml) and chemiluminescent detection substrate.

image 2 :

Detection of FOLR1 in MCF-7 human cell line. FOLR1 was detected in immersion fixed MCF-7 human breast cancer cell line using MBS605669 (10ug/ml) for 3 hours at RT. Cells were stained with the DayLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue).

image 3 :

Flow Cytometry detection of FOLR1 in MCF-7 human cell line using MBS605669. MCF-7 human breast cancer cell line was stained with MBS605669 (filled histogram) or isotype control antibody (open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody.

product information

catalog number :

MBS605669

products type :

Antibody

products full name :

products short name :

[FOLR1]

products name syn :

[Anti -FOLR1 (Folate receptor 1 (adult) Adult folate-binding protein, FBP, Folate receptor, adult, Folate receptor 1, Folate receptor alpha, FOLR, FR-alpha, KB cells FBP, MOv18, Ovarian tumor-associated antigen MOv18)]

other names :

[folate receptor alpha; Folate receptor alpha; folate receptor alpha; FR-alpha; KB cells FBP; folate binding protein; folate receptor, adult; adult folate-binding protein; ovarian tumor-associated antigen MOv18; folate receptor 1 (adult); Adult folate-binding protein; FBP; Folate receptor 1; Folate receptor, adult; KB cells FBP; Ovarian tumor-associated antigen MOv18]

other gene names :

[FOLR1; FOLR1; FBP; FOLR; FOLR; FR-alpha; FBP]

uniprot entry name :

FOLR1_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

[9H125]

host :

Mouse

reactivity :

Human

specificity :

Recognizes human FOLR1. In Direct ELISA, shows no crossreactivity with recombinant human FOLR2, 3 or 4.

purity :

Purified by Protein G affinity chromatography from hybridoma culture supernatant.

form :

Supplied as lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

concentration :

~0.5mg/ml (after reconstitution)

storage stability :

Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

tested application :

ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). Other applications not tested

app notes :

Recommended Dilution:. ELISA (Capture): 2-8ug/ml. Flow Cytometry: 0.25ug labels/10e6 cells. Tested in MCF-7 cells. Western Blot: 2ug/ml detects human FOLR1 in human cortex tissue and HeLa cells under non-reducing conditions only. Immunocytochemistry: 8-25ug/ml. Tested on immersion fixed MCF-7 cells. Optimal dilutions to be determined by the researcher.

image1 heading :

Western Blot (WB)

image2 heading :

Testing Data

image3 heading :

Flow Cytometry (FC/FACS)

other info1 :

Immunogen: Recombinant protein corresponding to Arg25-Met233 of human FOLR1 expressed in CHO cells (P15328). Species Sequence Homology: mouse, rat; 83%

other info2 :

Grade: Affinity Purified

products categories :

Antibodies; Abs to Binding Proteins

products description :

FOLR1 is a 37-42kD protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine and histidine breakdown. Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage. Human FOLR1 shares 83% amino acid sequence identity with mouse and rat FOLR1. FOLR1 is predominantly expressed on epithelial cells and is dramatically up- regulated on many carcinomas. It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects. FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface. A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk.

ncbi gi num :

9257213

ncbi acc num :

NP_057941.1

ncbi gb acc num :

NM_016729.2

uniprot acc num :

P15328

ncbi pathways :

Endocytosis Pathway (102279); Endocytosis Pathway (102181)

ncbi summary :

The protein encoded by this gene is a member of the folate receptor family. Members of this gene family bind folic acid and its reduced derivatives, and transport 5-methyltetrahydrofolate into cells. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. Mutations in this gene have been associated with neurodegeneration due to cerebral folate transport deficiency. Due to the presence of two promoters, multiple transcription start sites, and alternative splicing, multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Oct 2009]

uniprot summary :

FOLR1: Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD). NCFTD is an autosomal recessive disorder resulting from brain- specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy, and leukodystrophy. Recognition and diagnosis of this disorder is critical because folinic acid therapy can reverse the clinical symptoms and improve brain abnormalities and function. Belongs to the folate receptor family. Protein type: Membrane protein, GPI anchor. Chromosomal Location of Human Ortholog: 11q13.3-q14.1. Cellular Component: anchored to external side of plasma membrane; cell surface; clathrin-coated vesicle; membrane; brush border membrane; integral to plasma membrane; apical plasma membrane; plasma membrane; endosome. Molecular Function: folic acid transporter activity; receptor activity; folic acid binding. Biological Process: receptor-mediated endocytosis; folic acid metabolic process; folic acid transport. Disease: Neurodegeneration Due To Cerebral Folate Transport Deficiency

size1 :

0.1 mg

price1 :

695 USD

more info or order :

MyBioSource product webpage