Uroplakin III | MyBioSource MBS605111 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Uroplakin III

catalog :

MBS605111

quantity :

0.05 mg

price :

505 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, rat, cow

application :

western blot, immunohistochemistry

product information

catalog number :

MBS605111

products type :

Antibody

products full name :

Uroplakin III

products short name :

Uroplakin III

products name syn :

Anti -Uroplakin III

other names :

uroplakin-3a isoform 2; Uroplakin-3a; uroplakin-3a; uroplakin III; uroplakin 3A; Uroplakin III

other gene names :

UPK3A; UPK3A; UP3A; UPK3; UPIII; UPIIIA; UPK3; UP3a; UPIII

uniprot entry name :

UPK3A_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

5F161

host :

Host: Mouse; Source: Bovine

reactivity :

Bovine, Human, Porcine, Rat

specificity :

Recognizes bovine uroplakin III present in the superficial cell layer of the urothelium. Strongly stains the urothelial surface membrane in paraffin sections of human renal pelvis, ureter, bladder and urethra. Species Crossreactivity: human, porcine and rat

purity :

Affinity Purified. Purified by Protein A affinity chromatography.

form :

Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.

storage stability :

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Suitable for use in Western Blot and Immunohistochemistry. Not suitable for frozen sections. Dilution: Immunohistochemistry: 1:10 on paraffin-embedded tissue using a heat-induced retrieval method (e.g. microwave treatment.). Incubation Time: 1 hr at RT. Optimal dilution determined by the researcher.

other info1 :

Immunogen: Asymmetric unit membrane (AUM) preparation from bovine urinary bladder.

products categories :

Antibodies; Abs to Carbohydrates, Glycoproteins

products description :

The binding region of Uroplakin III has been localized on the extracellular part of the antigen. Together with the uroplakins UP Ia, UP Ib and UP II, uroplakin III contributes in constituting the asymmetrical unit membrane of the plaques of urothelial superficial (umbrella) cells. Uroplakin is a membrane glycoprotein (47kD) and has been shown to be a specific marker of terminal urothelial differentiation (Wu et al., 1993, 1994). About 60% of human transitional cell carcinomas (including metastases) maintain focal (sometimes very limited) expression of uroplakin III. Until now, no uroplakin staining has been found in any non-urothelial carcinoma (Moll et al., 1995). Uroplakin III may thus serve as a specific urothelial differentiation marker in cases of metastatic carcinomas with unclear primary tumor.

ncbi gi num :

266456217

ncbi acc num :

NP_001161046.1

ncbi gb acc num :

NM_001167574.1

uniprot acc num :

O75631

ncbi mol weight :

30,670 Da

ncbi summary :

This gene encodes a member of the uroplakin family, a group of transmembrane proteins that form complexes on the apical surface of the bladder epithelium. Mutations in this gene may be associated with renal adysplasia. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009]

uniprot summary :

Function: Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence . By similarity. Subunit structure: Heterodimer with uroplakin-1B (UPK1B) . By similarity. Subcellular location: Endoplasmic reticulum membrane; Single-pass type I membrane protein . By similarity. Note: Heterodimer formation with UPK1B is a prerequisite to exit out of the endoplasmic reticulum (ER) . By similarity. Tissue specificity: Expressed in ureter. Ref.6. Involvement in disease: Renal adysplasia (RADYS) [MIM:191830]: Renal agenesis refers to the absence of one (unilateral) or both (bilateral) kidneys at birth. Bilateral renal agenesis belongs to a group of perinatally lethal renal diseases, including severe bilateral renal dysplasia, unilateral renal agenesis with contralateral dysplasia and severe obstructive uropathy.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.7 Ref.8. Sequence similarities: Belongs to the uroplakin-3 family.

size :

0.05 mg

price :

505 USD