ADAMTS5 (Aggrecanase 2, A Disintegrin And Metalloproteinase with Thrombospondin-3 motif) | MyBioSource MBS605109 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ADAMTS5 (Aggrecanase 2, A Disintegrin And Metalloproteinase with Thrombospondin-3 motif)

catalog :

MBS605109

quantity :

0.1 mg

price :

655 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[9H41]

reactivity :

human

application :

western blot, ELISA, immunoprecipitation, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS605109

products type :

Antibody

products full name :

ADAMTS5 (Aggrecanase 2, A Disintegrin And Metalloproteinase with Thrombospondin-3 motif)

products short name :

[ADAMTS5]

products name syn :

[Anti -ADAMTS5 (Aggrecanase 2, A Disintegrin And Metalloproteinase with Thrombospondin-3 motif)]

other names :

[A disintegrin and metalloproteinase with thrombospondin motifs 5 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 5; A disintegrin and metalloproteinase with thrombospondin motifs 5; aggrecanase-2; OTTHUMP00000096353; a disintegrin and metalloproteinase with thrombospondin motifs 11; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2); ADAM metallopeptidase with thrombospondin type 1 motif, 5; A disintegrin and metalloproteinase with thrombospondin motifs 11; ADAM-TS 11; ADAMTS-11; ADMP-2; Aggrecanase-2]

other gene names :

[ADAMTS5; ADAMTS5; ADMP-2; ADAM-TS5; ADAMTS-5; ADAMTS11; FLJ36738; ADAM-TS 5; ADAMTS-11; ADAM-TS 11; ADAMTS11; ADMP2]

uniprot entry name :

ATS5_HUMAN

clonality :

Monoclonal

isotype :

IgG2b

clone :

[9H41]

host :

Mouse

reactivity :

Human

specificity :

Recognizes human ADAMTS5. In Western Blot, no cross-reactivity with recombinant human ADAMTS1 or recombinant human ADAMSTL 1.2 is observed.

purity :

Purified by Protein A affinity chromatography from hybridoma culture supernatant.

form :

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

concentration :

~0.5mg/ml (after reconstitution)

storage stability :

Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

tested application :

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)

app notes :

Dilution:. Western Blot: 1 ug/ml under non-reducing conditions. Immunoprecipitation: 25ug/ml using conditioned cell culture medium spiked with recombinant human ADAMTS5. Optimal dilutions to be determined by the researcher.

other info1 :

Grade: Affinity Purified. Immunogen: Recombinant protein corresponding to Ser262-Pro622 of human ADAMTS5, expressed in NSO cells.

products categories :

Antibodies; Abs to Matrix Metalloproteinases

products description :

ADAMTS5, also known as aggrecanase-2 and ADADMTS11, ADAMTS5 precursor consists of multiple domains. The purified rhADAMTS5 starts at the N-terminus of the catalytic domain and ends at the C-terminus of the TSP-1 domain. The amino acid sequence of rhADAMTS5 is 98%, 97%, and 96% identical to that of canine, bovine, and mouse/rat.

ncbi gi num :

195539372

ncbi acc num :

NP_008969.2

ncbi gb acc num :

NM_007038.3

uniprot acc num :

Q9UNA0

ncbi mol weight :

101,718 Da

ncbi pathways :

Endochondral Ossification Pathway (198812)

ncbi summary :

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene contains two C-terminal TS motifs and functions as aggrecanase to cleave aggrecan, a major proteoglycan of cartilage. [provided by RefSeq]

uniprot summary :

ADAMTS5: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period. Protein type: Motility/polarity/chemotaxis; EC 3.4.24.-; Extracellular matrix; Protease; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 21q21.3. Cellular Component: proteinaceous extracellular matrix; endoplasmic reticulum lumen; extracellular region. Molecular Function: integrin binding; protein binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity. Biological Process: extracellular matrix disassembly; protein amino acid O-linked glycosylation; extracellular matrix organization and biogenesis; cellular protein metabolic process; defense response to bacterium; proteolysis; post-translational protein modification

size1 :

0.1 mg

price1 :

655 USD

more info or order :

MyBioSource product webpage