antibody

Tau, 4-repeat isoform RD4 (Tau Protein, Microtubule-associated Protein, MAP)

MBS604301

0.2 mL

760 USD

monoclonal

mouse

nonconjugated

[4H380]

Antibody

Tau, 4-repeat isoform RD4 (Tau Protein, Microtubule-associated Protein, MAP)

[Tau, 4-repeat isoform RD4]

[Anti -Tau, 4-repeat isoform RD4 (Tau Protein, Microtubule-associated Protein, MAP)]

Monoclonal

IgG

[4H380]

Mouse

Species Crossreactivity: Rat. Species Sequence Homology: Mouse and Bovine.

Recognizes human Tau (4-repeat isoform RD4), Mr 45-65kD. Higher MW band (68-72kD) represents phosphorylated Tau.

Supernatant

Supplied as a liquid, 0.05% sodium azide. Also available without azide. See MBS603757 .

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB), Immunohistochemistry (IHC). Other applications not tested

Recommended Dilution:. Western Blot: 1:1000-1:10,000. Detects Tau (4-repeat isoform RD4) in a rat brain cytosolic fraction. Rat brain cytosol fraction was resolved by electrophoresis, transferred to nitrocellulose and probed with MBS604301. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Immunohistochemistry: Detects Tau (4-repeat isoform RD4) in autoclaved paraffin brain sections (1-2). Differential Detection of Tauopathies: See reference 2. Optimal dilutions to be determined by the researcher.

Western Blot (WB)

Immunogen: Synthetic peptide corresponding to aa275-291, VQIINKKLDLSNVQSKC of human Tau (4-repeat isoform RD4) (Bovine thyroglobulin coupled). Region is flanking junction coded by adjacent exons 9 & 11 with the inclusion of exon 10.!

Westem Blot Protocol: 1. Perform SDS-PAGE on a cell lysate sample (cell lysis buffer: SOmM Tris-HCI, pH 7.4; 1 % NP-40; 0.2S% sodium deoxycholate; lS0mM sodium chloride; 1 mM EDTA; 1 mM PMSF; 1 ugl,ml each aprotinin, leupeptin, pepstatin; 1 mM Na3V04, 1 mM NaF). Transfer the proteins to nitrocellulose. Wash the blotted nitrocellulose twice with water. 2. Block the bl9tted nitrocellulose in freshly prepared TBS, S% nonfat dry milk, O.O5% Tween-20 (TBST-MLK) for 2 hours at RT with constant agitation. 3. Incubate the nitrocellulose with a 1: 1 000-1: 1 0,000 dilution of T1 030-40A anti-Tau (4-repeat isoform RD4), diluted in freshly prepared TBST-MLK overnight with agitation at 4 degree C . 4. Wash the nitrocellulose twice with water. S. Incubate the nitrocellulose in the secondary reagent of choice (lgG (HRP) goat anti-mouse)* in TBST-MLK for 1-2 hours with agitation at RT. 6. Wash the nitrocellulose twice with water. 7. Wash the nitrocellulose in TBS, O.OS% Tween-20 for lS minutes. 8. Rinse the nitrocellulose in 4-S changes of water. 9. Rinse the nitrocellulose in TBS with agitation for about an hour at RT. 10. Use detection method of choice. Ennanced chemiluminescence was used. Recommended Second Antibodies: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo . IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo

Antibodies; Abs to Microtubule-associated Proteins

Microtubule Associated Proteins (MAPS) bind to the tubulin subunits of microtubule structures and regulate their functional stability. In the cell MAPs bind to monomer and multimerized tubulin. MAP binding to multimerized tubulin further stabilizes the formation of higher order microtubulin structures. MAP binding to microtubule structures is mediated through phosphorylation through Microtubule Affinity Regulated Kinase (MARK). Phosphorylation releases MAPs bound to microtubules, destabilizing the structure, driving it toward disassembly. There are predominately two MAP types, I, II. Type II MAP includes MAP2, MAP4 and tau,and are found in nervous tissue. Six tau isoforms exist in brain tissue; they are distinguished by their number of binding domains. Three isoforms have three binding domains and the other three have four binding domains. The binding domains are located in the carboxy-terminus of the protein and are positively-charged. The isoforms with four binding domains are better at stabilizing microtubules than those with three binding domains.

NP_001116538

0.2 mL

760 USD