antibody

MCAM (Melanoma Cell Adhesion Molecule) (PE)

MBS604218

0.1 mg

485 USD

monoclonal

mouse

PE

[5K96]

human, chicken

flow cytometry, other

Antibody

MCAM (Melanoma Cell Adhesion Molecule) (PE)

[MCAM]

[Anti -MCAM (Melanoma Cell Adhesion Molecule) (PE)]

[Melanoma cell adhesion molecule; Cell surface glycoprotein MUC18; cell surface glycoprotein MUC18; Gicerin; OTTHUMP00000232151; melanoma adhesion molecule; cell surface glycoprotein P1H12; melanoma-associated antigen A32; melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen; melanoma cell adhesion molecule; Cell surface glycoprotein P1H12; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen]

[MCAM; MCAM; CD146; MUC18; MUC18]

MUC18_HUMAN

Monoclonal

IgG2b,k

[5K96]

Mouse

Chicken

Recognizes chicken MCAM, Mr 98kD (protein core ~84kD).

Purified

Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE.

0.1 mg/mL

Store product a 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as a undiluted liquid. Dilute required amount only prior to inmediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light . For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Suitable for use in Flow Cytometry. Other applications not tested.

Flow Cytometry: 0.2 ug/10e6 cells. Optimal dilutions to be determined by the researcher.

Flow Cytometry (FC/FACS)

Immunogen: Pooled Ed15 and Ed16 thymocytes from H.B15, H.B19 and H.B21 chicken embryos

Antibodies; Abs to Cancer Markers

Chicken MCAM, a member of the immunoglobulin superfamily of cell surface receptors, consists of the V-V-C2-C2-C2 Ig domains. It is a single chain glycoprotein with three mRNA splice variants. One has a short cytoplasmic tail, another has a long tail, and the third seems to lack transmembrane and cytoplasmic regions. It is highly homologous to gicerin, a molecule involved in neurite outgrowth and Wilm's kidney tumor progression in the chicken, and to the human melanoma progression molecule MUC18. Chicken MCAM is expressed by c-kit+ hematopoietic progenitor cells in embryonic bone marrow, thymocytes, and capillary endothelial cells. MCAM-expressing cells sorted from bone marrow are enriched in progenitors capable of differentiating into T cells when transferred into the appropriate thymic microenvironment. MCAM has been proposed to be involved in cellular adhesion and homing processes.1

33989399

P43121

MCAM: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell adhesion; Membrane protein, integral. Chromosomal Location of Human Ortholog: 11q23.3. Cellular Component: extracellular space; focal adhesion; integral to membrane; plasma membrane; nucleus; external side of plasma membrane. Biological Process: anatomical structure morphogenesis; glomerular filtration; angiogenesis; cell adhesion; positive regulation of cell migration

0.1 mg

485 USD