antibody

HLA Class 2 Antigen DR4 (X-Reactive)

MBS604087

0.1 mL

505 USD

monoclonal

mouse

nonconjugated

[8.L.335]

Antibody

HLA Class 2 Antigen DR4 (X-Reactive)

[HLA Class 2 Antigen DR4]

[Anti -HLA Class 2 Antigen DR4 (X-Reactive)]

[HLA-A11 antigen A11.2; HLA class I histocompatibility antigen, A-68 alpha chain; HLA class I histocompatibility antigen, A-1 alpha chain; antigen presenting molecule; leukocyte antigen class I-A; MHC class I antigen HLA-A heavy chain; major histocompatibility complex, class I, A; Aw-68; HLA class I histocompatibility antigen, A-28 alpha chain; MHC class I antigen A*68]

[HLA-A; HLA-A; HLAA; HLAA]

1A68_HUMAN

Monoclonal

IgM

[8.L.335]

Mouse

Human

Recognizes HLA Class 2 Antigen-DR4. Additional crossreactivity occurs with related proteins. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.

Ascites

Supplied as a lyophilized powder from PBS, 1% BSA. Reconstitute with 100ul sterile ddH2O to make a 10X stock solution.

Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

This antibody has not been tested in methodologies other than Microcytoxicity Test. Potential applications include Flow Cytometry, Cell Typing, Tissue Staining and Chimerism Studies. Other applications not tested.

Cytotoxicity: Add 0.9ml of 1% BSA in PBS to 100ul of 10X working dilution. optimal dilutions to be determined by the researcher

Cytotoxicity: Expected Results:. Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction. Limitations:. Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.

Source: Ascites fluid

Antibodies; Abs to HLA

487911

BAA04118.1

P01891

Adaptive Immune System Pathway (366160); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway (366163); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Antigen Processing-Cross Presentation Pathway (477122); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Cell Adhesion Molecules (CAMs) Pathway (83069)

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008]

HLAA: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family. Protein type: Immunoglobulin superfamily; Membrane protein, integral. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: Golgi membrane; Golgi apparatus; phagocytic vesicle membrane; cell surface; membrane; early endosome membrane; endoplasmic reticulum; integral to plasma membrane; plasma membrane; MHC class I protein complex; Golgi medial cisterna. Molecular Function: protein binding; peptide antigen binding; beta-2-microglobulin binding; T cell receptor binding; TAP binding; receptor binding. Biological Process: regulation of immune response; detection of bacterium; viral reproduction; positive regulation of T cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; protection from natural killer cell mediated cytotoxicity; regulation of defense response to virus by virus; positive regulation of interferon-gamma production; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent; antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I; immune response. Disease: Severe Cutaneous Adverse Reaction, Susceptibility To

0.1 mL

505 USD