Heparin Binding EGF-like Growth Factor (HB-EGF, HBEGF, Diphtheria Toxin Receptor, DT-R, DTR) | MyBioSource MBS603983 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Heparin Binding EGF-like Growth Factor (HB-EGF, HBEGF, Diphtheria Toxin Receptor, DT-R, DTR)

catalog :

MBS603983

quantity :

0.5 mg

price :

660 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7H229

reactivity :

human

application :

western blot, ELISA, neutralization, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS603983

products type :

Antibody

products full name :

Heparin Binding EGF-like Growth Factor (HB-EGF, HBEGF, Diphtheria Toxin Receptor, DT-R, DTR)

products short name :

Heparin Binding EGF-like Growth Factor

products name syn :

Anti -Heparin Binding EGF-like Growth Factor (HB-EGF, HBEGF, Diphtheria Toxin Receptor, DT-R, DTR)

other names :

heparin-binding EGF-like growth factor; Proheparin-binding EGF-like growth factor; proheparin-binding EGF-like growth factor; OTTHUMP00000159876; heparin-binding epidermal growth factor; diphtheria toxin receptor (heparin-binding EGF-like growth factor); diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor); heparin-binding EGF-like growth factor; Diphtheria toxin receptor

other gene names :

HBEGF; HBEGF; DTR; DTS; DTSF; HEGFL; DTR; DTS; HEGFL

uniprot entry name :

HBEGF_HUMAN

clonality :

Monoclonal

isotype :

IgG2a

clone :

7H229

host :

Mouse

reactivity :

Human

specificity :

Recognizes human Heparin Binding EGF-like Growth Factor (HB-EGF). Does not crossreact with recombinant human Amphiregulin, BTC, HRG-alpha, HRG-beta, EGF, TGF-alpha or recombinant mouse Epigen or Epiregulin.

purity :

Affinity Purified. Purified by Protein G affinity chromatography.

form :

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.

concentration :

~0.5 mg/ml (after reconstitution)

storage stability :

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

tested application :

ELISA (EL/EIA), Western Blot (WB), Neutralization

app notes :

Suitable for use in Western Blot, Neutralization and ELISA. Dilution: Western Blot: 1ug/ml using recombinant human Heparin Binding EGF-like Growth Factor. Neutralization: ~0.5-2.5ug/ml yields one-half maximal inhibition (ND50) of the biological activity of recombinant human HB-EGF (5ng/ml). Measured by its ability to neutralize HB-EGF-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line.

other info1 :

Immunogen: Recombinant protein corresponding to aa63-148 of human HB-EGF expressed in Sf21. Species Sequence Homology (77%): rat and mouse. Endotoxin Level: <0.1EU/ug (LAL).

products categories :

Antibodies; Abs to Growth Factors, Cytokines

products description :

Heparin-binding EGF-like Growth Factor (HB-EGF) is an EGF family growth factor that is synthesized as a transmembrane precursor (proHB-EGF). ProHB-EGF, which contains one EGF-like domain, is subject to variable O-linked glycosylation and N-terminal truncation. In human, this precursor is the cellular receptor for diphtheria toxin. ADAM12 or ADAM17-mediated cleavage of proHB-EGF releases a 22 kD soluble HB-EGF; the cytoplasmic fragment functions as a transcriptional repressor. HB-EGF directly activates EGF R and ErbB4 and promotes the development of many tissues. Human HB-EGF shares 77% aa sequence identity with mouse and rat HB-EGF.

ncbi gi num :

183867

uniprot acc num :

Q99075

ncbi mol weight :

23,067 Da

ncbi pathways :

Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway (83102); Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway (515); ErbB Receptor Signaling Network Pathway (138016); ErbB Signaling Pathway (198844); ErbB Signaling Pathway (83049); ErbB Signaling Pathway (458); ErbB4 Signaling Events Pathway (137991); GnRH Signaling Pathway (83091); GnRH Signaling Pathway (502); Hypertrophy Model Pathway (198828)

uniprot summary :

HBEGF: Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor. Protein type: Membrane protein, integral; Cell surface. Chromosomal Location of Human Ortholog: 5q23. Cellular Component: extracellular space; cell surface; integral to plasma membrane; plasma membrane; extracellular region. Molecular Function: heparin binding; growth factor activity; epidermal growth factor receptor binding. Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of keratinocyte migration; muscle development; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; positive regulation of smooth muscle cell proliferation; negative regulation of elastin biosynthetic process; pathogenesis; signal transduction; positive regulation of cell growth; regulation of heart contraction; positive regulation of protein kinase B signaling cascade; positive regulation of cell proliferation; innate immune response; wound healing, spreading of epidermal cells; positive regulation of cell migration

size1 :

0.5 mg

price1 :

660 USD

more info or order :

MyBioSource product webpage