catalog number :
MBS603901
products full name :
Mouse anti-Human HLA Class 1 Antigen A33,B8
products short name :
[HLA Class 1 Antigen A33,B8]
products name syn :
[Anti -HLA Class 1 Antigen A33,B8]
specificity :
Recognizes HLA Class 1 Antigen A33,B8. Specificity was determined at a 1:10 dilution by the Microcytotoxicity Assay under standard NIH conditions.
form :
Supplied as a lyophilized powder from PBS, 1% BSA. Reconstitute with 100ul sterile ddH2O to make a 10X stock solution.
storage stability :
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Recon- stituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
tested application :
This antibody has not been tested in methodologies other than Microcytotoxicity Test. Potential applications include Flow Cytometry, Cell Typing, Tissue Staining and Chimerism Studies.
app notes :
Recommended Dilutions:. Cytotoxicity: Add 0.9ml of 1% BSA in PBS to 100ul of 10X working dilution.
Optimal dilutions to be determined by the researcher.
image1 heading :
Testing Data
other info1 :
Cytotoxicity: Expected Results: . Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction. Limitations: . Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.
products categories :
Antibodies; Abs to HLA
products description :
The human leukocyte antigen system (HLA) is the name of the major histocompatibility complex (MHC) in humans and is an essential element for immune function. This group of genes re- sides on chromosome 6, and encodes cell-surface antigen- presenting proteins and many other genes. Different classes have different functions: HLA class I antigens (A, B & C) present peptides from inside the cell, including viral peptides if present. These peptides are produced from digested proteins that are broken down in the proteasomes. Foreign antigens attract killer T-cells (also called CD8+ or cytotoxic T-cells) that destroy cells.