antibody

Transforming Growth Factor beta1, beta2, beta3 (TGFb1, TGFb2, TGFb3) (APC)

MBS603833

100 Tests

690 USD

monoclonal

mouse

APC

7H7

human, mouse, cow

flow cytometry, FACS

Antibody

Transforming Growth Factor beta1, beta2, beta3 (TGFb1, TGFb2, TGFb3) (APC)

Transforming Growth Factor beta1, beta2, beta3

Mouse Anti-Bovine Transforming Growth Factor beta1, beta2, beta3 (TGFb1, TGFb2, TGFb3) (APC)

transforming growth factor beta-1; Transforming growth factor beta-1; transforming growth factor beta-1; TGF-beta-1; TGF-beta 1 protein; latency-associated peptide; transforming growth factor, beta 1

TGFB1; TGFB1; CED; LAP; DPD1; TGFB; TGFbeta; TGFB

TGFB1_HUMAN

Monoclonal

IgG1

7H7

Mouse

Avian, Bovine, Chicken, Human, Mouse

Recognizes bovine TGFb1 and TGFb2. Species Crossreactivity: mouse TGFb1 and TGFb2; rhTGFb1, rhTGFb2 and rhTGFb3; recombinant chicken TGFb3

Affinity Purified. Purified by Protein G affinity chromatography.

Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).

Store product at 4 degree C in the dark. Do not freeze! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensityve to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Flow Cytometry (FC/FACS)

Suitable for use in Flow Cytometry. Dilution: Flow Cytometry: Neat. Use 10ul to label 5x10e6 cells. For intracellular staining, PC-3 human prostate cancer cell line must be fixed with Flow Cytometry Fixation Buffer and permeadbilized with Flow Cytometry Permeabilization/Wash Buffer. Optimal dilutions to be determined by the researcher.

Immunogen: Bovine bone-derived TGFb1 and TGFb2

Antibodies; Abs to Growth Factors, Cytokines

Transforming Growth Factor Beta (TGFb) is a stable, multifunctional polypeptide growth factor. While specific receptors for this protein have been found on almost all mammalian cell types thus far examined, the effect of the molecule varies depending on the cell type and growth conditions. Generally, TGFb is stimulatory for cells of mesenchymal origin and inhibitory for cells of epithelial or neuroectodermal origin. TGFb1, 2 and 3 are a closely related groupd of proteins (70-80%) sequence homology) that are produced by many cell types and function as growth and differentiation factors. the active forms of TGFb1, 2, and 3 are disulfide-linked homdimers.

63025222

NP_000651.3

NM_000660.4

P01137

44,341 Da

ACE Inhibitor Pathway (198763); ALK1 Signaling Events Pathway (137968); Adipogenesis Pathway (198832); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795)

This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.

TGFB1: Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Homodimer; disulfide-linked, or heterodimer with TGFB2. Secreted and stored as a biologically inactive form in the extracellular matrix in a 290 kDa complex (large latent TGF-beta1 complex) containing the TGFB1 homodimer, the latency-associated peptide (LAP), and the latent TGFB1 binding protein-1 (LTBP1). The complex without LTBP1 is known as the small latent TGF-beta1 complex . Dissociation of the TGFB1 from LAP is required for growth factor activation and biological activity. Release of the large latent TGF-beta1 complex from the extracellular matrix is carried out by the matrix metalloproteinase MMP3. May interact with THSD4; this interaction may lead to sequestration by FBN1 microfibril assembly and attenuation of TGFB signaling. Interacts with the serine proteases, HTRA1 and HTRA3: the interaction with either inhibits TGFB1-mediated signaling. The HTRA protease activity is required for this inhibition. Interacts with CD109, DPT and ASPN. Activated in vitro at pH below 3.5 and over 12.5. Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage. Belongs to the TGF-beta family. Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 19q13.1. Cellular Component: proteinaceous extracellular matrix; extracellular space; microvillus; cell surface; cell soma; axon; Golgi lumen; cytoplasm; extracellular region; plasma membrane; nucleus. Molecular Function: protein binding; enzyme binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; punt binding; cytokine activity; protein N-terminus binding; glycoprotein binding; antigen binding. Biological Process: extracellular matrix organization and biogenesis; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; SMAD protein nuclear translocation; female pregnancy; positive regulation of protein amino acid dephosphorylation; activation of NF-kappaB transcription factor; regulation of protein import into nucleus; positive regulation of MAP kinase activity; connective tissue replacement during inflammatory response; regulation of transforming growth factor beta receptor signaling pathway; negative regulation of ossification; cell cycle arrest; positive regulation of isotype switching to IgA isotypes; inner ear development; regulatory T cell differentiation; positive regulation of interleukin-17 production; response to drug; positive regulation of smooth muscle cell differentiation; positive regulation of chemotaxis; active induction of host immune response by virus; positive regulation of blood vessel endothelial cell migration; regulation of sodium ion transport; negative regulation of fat cell differentiation; negative regulation of blood vessel endothelial cell migration; lymph node development; positive regulation of protein secretion; positive regulation of transcription from RNA polymerase II promoter; response to progesterone stimulus; endoderm development; positive regulation of odontogenesis; myelination; negative regulation of phagocytosis; evasion of host defenses by virus; positive regulation of cellular protein metabolic process; myeloid dendritic cell differentiation; negative regulation of transcription from RNA polymerase II promoter; phosphate metabolic process; negative regulation of cell proliferation; negative regulation of T cell proliferation; regulation of DNA binding; ureteric bud development; negative regulation of release of sequestered calcium ion into cytosol; salivary gland morphogenesis; positive regulation of cell proliferation; protein kinase B signaling cascade; protein export from nucleus; inflammatory response; positive regulation of exit from mitosis; aging; epidermal growth factor receptor signaling pathway; mitotic cell cycle checkpoint; common-partner SMAD protein phosphorylation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of bone mineralization; positive regulation of peptidyl-serine phosphorylation; SMAD protein complex assembly; positive regulation of protein kinase B signaling cascade; positive regulation of protein complex assembly; positive regulation of protein import into nucleus; response to hypoxia; epithelial to mesenchymal transition; negative regulation of cell-cell adhesion; negative regulation of cell growth; negative regulation of transforming growth factor beta receptor signaling pathway; negative regulation of skeletal muscle development; mononuclear cell proliferation; regulation of cell migration; protein amino acid phosphorylation; hyaluronan catabolic process; regulation of apoptosis; response to vitamin D; negative regulation of neuroblast proliferation; transforming growth factor beta receptor signaling pathway; receptor catabolic process; positive regulation of superoxide release; germ cell migration; response to glucose stimulus; chondrocyte differentiation; negative regulation of mitotic cell cycle; T cell homeostasis; defense response to fungus, incompatible interaction; cell growth; tolerance induction to self antigen; regulation of striated muscle development; platelet activation; organ regeneration; negative regulation of DNA replication; virus-host interaction; hemopoietic progenitor cell differentiation; negative regulation of transcription, DNA-dependent; positive regulation of epithelial cell proliferation; positive regulation of collagen biosynthetic process; viral infectious cycle; response to estradiol stimulus; negative regulation of cell cycle; positive regulation of histone deacetylation; response to radiation; platelet degranulation; negative regulation of protein amino acid phosphorylation; lipopolysaccharide-mediated signaling pathway; response to wounding; adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains; negative regulation of epithelial cell proliferation; intercellular junction assembly and maintenance; regulation of binding; MAPKKK cascade; cellular calcium ion homeostasis; gut development; protein import into nucleus, translocation; ATP biosynthetic process; positive regulation of histone acetylation; positive regulation of protein amino acid phosphorylation; blood coagulation; negative regulation of myoblast differentiation; positive regulation of cell migration. Disease: Camurati-engelmann Disease; Cystic Fibrosis

100 Tests

690 USD