catalog number :
MBS603723
products full name :
West Nile Virus, NS3 Protease (WNV)
products short name :
West Nile Virus, NS3 Protease
products name syn :
Anti -West Nile Virus, NS3 Protease (WNV)
specificity :
Recognizes west nile virus NS3 protease.
purity :
Affinity Purified. Purified by Protein G affinity chromatography.
form :
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitution: Reconstitute with 200ul sterile PBS.
concentration :
~0.5mg/ml (after reconstitution)
storage stability :
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
tested application :
ELISA (EL/EIA), Western Blot (WB)
app notes :
Western Blot, Direct ELISA. Dilution: Western Blot: This antibody can be used at 1-2ug/mL with the appropriate secondary reagents to detect west nile virus NS3 protease. Using a colorimetric detection system, the detection limit for rwnvNS3 protease is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Direct ELISA: This antibody can be used at 0.5-1.0ug/mL with the appropriate secondary reagents to detect west nile virus NS3 protease. The detection limit for rwnvNS3 is approximately 2 ng/well.
other info1 :
Immunogen: Recombinant protein corresponding aa1-184 of NS3 (NP_776018) and aa49-96 of NS2b (NP_776017) of West Nile Virus NS3 Protease, express in E.coli.
other info2 :
Hybridoma: Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, E.coli-derived, recombinant West Nile Virus NS3 Protease expressed as a fusion with the cofactor NS2b, (rwnvNS3, aa 1-184 of NS3, Accession # NP_776018, and aa 49-96 of NS2b, Accession # NP_776017).
products categories :
Antibodies; Abs to Infectious Disease WNV
products description :
The trypsin-like protease, NS3, and its co-factor, NS2b, play an essential role in replication and are therefore potential targets for treatment and prevention of West Nile Virus disease.