catalog number :
MBS603700
products full name :
HLA Class 1 Antigen B35,B57,B75,B77(X-Reactive)
products short name :
[HLA Class 1 Antigen B35,B57,B75,B77(X-Reactive)]
products name syn :
[Anti -HLA Class 1 Antigen B35,B57,B75,B77(X-Reactive)]
specificity :
Recognizes HLA Class 1 Antigen-B35,B57,B75,B77. Additional crossreactivity occurs with related proteins. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.
form :
Supplied as a lyphilized powder. Reconstitute with 100ul sterile ddH2O to make a 10X stock solution.
storage stability :
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
tested application :
This antibody has not been tested in methodologies other than Microcytotoxicity Test. Potential applications included Flow Cytometry, Cell Typing, Tissue Staining and Chimerism studies.
app notes :
Suitable for use in Cell Typing, Tissue Staining and Chimerism Studies. Dilution: . Cytotoxic Working Dilution: Add 0.9ml 1% BSA in PBS to 100ul of10X stock solution. Optimal dilutions to be determined by the researcher.
other info1 :
Grade: Ascites. Immunogen: B cells expressing HLA class I antigen
other info2 :
Expected Results: Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction. Limitations: Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.
products categories :
Antibodies; Abs to HLA