antibody

cdk1, p34cdc2 (CDC2, Cell Division Cycle 2, G1 to S and G2 to M, CDC28A, CDK1, Cell division control protein 2 homolog, Cyclin-dependent kinase 1, MGC111195, p34 protein kinase, DKFZp686L20222)

MBS603644

0.05 mg

620 USD

monoclonal

mouse

nonconjugated

[2Q305]

human, mouse, rat, chicken

Antibody

cdk1, p34cdc2 (CDC2, Cell Division Cycle 2, G1 to S and G2 to M, CDC28A, CDK1, Cell division control protein 2 homolog, Cyclin-dependent kinase 1, MGC111195, p34 protein kinase, DKFZp686L20222)

[cdk1, p34cdc2]

[Anti -cdk1, p34cdc2 (CDC2, Cell Division Cycle 2, G1 to S and G2 to M, CDC28A, CDK1, Cell division control protein 2 homolog, Cyclin-dependent kinase 1, MGC111195, p34 protein kinase, DKFZp686L20222)]

[Cyclin-dependent kinase 1; Cyclin-dependent kinase 1; cyclin-dependent kinase 1; OTTHUMP00000019659; OTTHUMP00000019660; OTTHUMP00000223862; p34 protein kinase; cell cycle controller CDC2; cell division protein kinase 1; cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M; cyclin-dependent kinase 1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase]

[CDK1; CDK1; CDC2; CDC28A; P34CDC2; MGC111195; DKFZp686L20222; CDC2]

CDK1_HUMAN

Monoclonal

IgG2a

[2Q305]

Mouse

human,mouse,rat,guinea pig,woodchuck and chicken.

Recognizes Xenopus cdk1/p34cdc2 protein kinase at ~34kD. Does not crossreact with cdk2/p32 and other cyclin dependent kinases (cdks). Epitope maps near the C-terminus of protein and its core is thought to be LGTPNNEV. Inhibits the activation of p43cdc2 kinase by cyclins and precipitates an active kinase.

Purified

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.

0.2 mg/ml

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF), Kinase Assay. Other applications have not been tested.

Recommended Dilution:. Immunoprecipitation (Native, denatured): 2ug/mg protein lysate using Protein A. Immunohistochemistry (formalin/paraffin, frozen): 2-4ug/ml for 30 minutes at RT. Paraffin sections require antigen retrieval. Western Blot: 2ug/ml for 2 hours at RT. Optimal dilution determined by the researcher.

Immunogen: Xenopus p34cdc2. Cellular Localization: Nuclear with some cytoplasmic staining.

Positive Control: HeLa or NIH3T3 cells; germinal center in a tonsil or reactive lymph node.

Antibodies; Abs to Cell Division Cycle (CDC)

Cdk1/p34cdc2 protein kinase is a catalytic subunit of Maturation Promoting Factor. p34cdc2, a serine/threonine kinase, is activated by cyclins, presumably by dephosphorylation of tyrosine residues.

334302921

P06493

APC/C-mediated Degradation Of Cell Cycle Proteins Pathway (105825); APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway (105832); APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway (105831); ARMS-mediated Activation Pathway (106466); Activated TLR4 Signalling Pathway (106400); Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway (105829); Androgen Receptor Signaling Pathway (198806); Axon Guidance Pathway (105688); Cell Cycle Checkpoints Pathway (105739); Cell Cycle, Mitotic Pathway (105765)

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

CDK1: a protein kinase of the CDK family. Catalytic subunit of the conserved protein complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions. Mitotic cyclins stably associate with this protein and function as regulatory subunits. Its activity is controlled by cyclin availability and phosphorylation through the cell cycle. Activated in many cancers including colon, liver and breast. The T isoform, which lacks a regulatory region, is expressed in breast cancer. Inhibition in cancer cells may drive cells into apoptosis. May also drive cell migration. Inhibitors: BMS-265246, BMS-265246-01. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Kinase, protein; Motility/polarity/chemotaxis; Cell cycle regulation; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.22; EC 2.7.11.23; CMGC group; CDK family; CDK/CDK1 subfamily; CDK1 subfamily. Chromosomal Location of Human Ortholog: 10q21.1. Cellular Component: nucleoplasm; centrosome; membrane; mitochondrion; spindle microtubule; cytoplasm; midbody; cytosol; nucleus. Molecular Function: RNA polymerase subunit kinase activity; protein serine/threonine kinase activity; cyclin binding; protein binding; cyclin-dependent protein kinase activity; histone kinase activity; Hsp70 protein binding; ATP binding; protein kinase activity. Biological Process: regulation of Schwann cell differentiation; nerve growth factor receptor signaling pathway; activation of MAPKK activity; activation of MAPK activity; response to toxin; histone phosphorylation; regulation of embryonic development; ventricular cardiac muscle cell development; stress-activated MAPK cascade; centrosome cycle; toll-like receptor 3 signaling pathway; response to organic cyclic substance; toll-like receptor 5 signaling pathway; small GTPase mediated signal transduction; protein complex assembly; G2/M transition of mitotic cell cycle; toll-like receptor 4 signaling pathway; positive regulation of cardiac muscle cell proliferation; response to drug; mitosis; organ regeneration; fibroblast growth factor receptor signaling pathway; positive regulation of protein import into nucleus, translocation; cell aging; pronuclear fusion; toll-like receptor 2 signaling pathway; chromosome condensation; response to ethanol; cell division; response to activity; toll-like receptor 9 signaling pathway; negative regulation of apoptosis; G1/S transition of mitotic cell cycle; response to amine stimulus; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; axon guidance; apoptosis; mitotic nuclear envelope disassembly; positive regulation of mitotic cell cycle; toll-like receptor 10 signaling pathway; epithelial cell differentiation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; response to axon injury; DNA replication; epidermal growth factor receptor signaling pathway; cell migration; organelle organization and biogenesis; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; peptidyl-threonine phosphorylation; microtubule cytoskeleton organization and biogenesis; DNA repair; MyD88-dependent toll-like receptor signaling pathway; G1/S-specific transcription in mitotic cell cycle; peptidyl-serine phosphorylation; response to cadmium ion; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; response to copper ion; mitotic cell cycle G2/M transition DNA damage checkpoint; Ras protein signal transduction; toll-like receptor signaling pathway; insulin receptor signaling pathway; innate immune response; mitotic cell cycle; vascular endothelial growth factor receptor signaling pathway; positive regulation of DNA replication

0.05 mg

620 USD