antibody

14-3-3 Binding Motif, phosphorylated (Ser)

MBS603373

0.1 mL

395 USD

monoclonal

mouse

nonconjugated

3i2

western blot, ELISA, immunoprecipitation, enzyme immunoassay

Antibody

14-3-3 Binding Motif, phosphorylated (Ser)

14-3-3 Binding Motif

Anti -14-3-3 Binding Motif, phosphorylated (Ser)

14-3-3 protein beta/alpha; 14-3-3 protein beta/alpha; 14-3-3 protein beta/alpha; 14-3-3 alpha; protein 1054; brain protein 14-3-3, beta isoform; protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide; Protein 1054; Protein kinase C inhibitor protein 1

YWHAB; YWHAB; HS1; GW128; YWHAA; KCIP-1; KCIP-1

1433B_HUMAN

Monoclonal

IgG1

3i2

Mouse

Mammal

Recognizes peptides/proteins containing phospho-Ser surrounded by Pro at the +2 position and Arg/Lys at the -3 position. By ELISA, recognizes a wide range of peptides containing this phosphorylated 14-3-3 binding motif in a manner that is phospho-specific and largely independent of the surrounding amino acid sequence. Weakly crossreacts with sequences containing phospho-Thr instead of phospho-Ser in this motif and with sequences containing phospho-Ser surrounded by Phe at the +1 position and Arg/Lys at the -3 position. No crossreactivity is observed with corresponding nonphosphorylated sequences.

Ascites. Ascites

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)

Suitable for use in ELISA, Western Blot and Immunoprecipitation. Dilution: Peptide ELISA: 1:1000. Western Blot: 1:4000. Immunoprecipitation: 1:20 (Denatured proteins).

Immunogen: Synthetic phospho-(Ser) 14-3-3 binding motif peptides (KLH).

Antibodies; Abs to 14-3-3

The 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control (1-5). Biological regulation by 14-3-3 is mediated through phosphorylation-dependent protein-protein interactions (6). Two different phospho-Ser-containing motifs are found within nearly all known 14-3-3 binding proteins (7). Motif 1 (Arg/Lys and Ser at positions -3 and -2, phospho-Ser at position 0, and Pro at position +2) is found in critical regulatory proteins including Bad, cdc25c, FKHRL1, PKC and c-Raf (5,7).

21328448

NP_647539.1

NM_139323.3

P31946

28,082 Da

ARMS-mediated Activation Pathway (106466); Activation Of BAD And Translocation To Mitochondria Pathway (105659); Activation Of BH3-only Proteins Pathway (105658); Adaptive Immune System Pathway (366160); Alpha6-Beta4 Integrin Signaling Pathway (198807); Apoptosis Pathway (105648); Axon Guidance Pathway (105688); Calcium Regulation In The Cardiac Cell Pathway (198906); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054)

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

14-3-3 beta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 20q13.1. Cellular Component: focal adhesion; cytoplasmic vesicle membrane; membrane; transcriptional repressor complex; perinuclear region of cytoplasm; cytoplasm; melanosome; cytosol; nucleus. Molecular Function: protein C-terminus binding; protein domain specific binding; protein binding; enzyme binding; histone deacetylase binding; protein complex binding; phosphoprotein binding; transcription corepressor activity; phosphoserine binding. Biological Process: positive regulation of catalytic activity; negative regulation of protein amino acid dephosphorylation; epidermal growth factor receptor signaling pathway; axon guidance; fibroblast growth factor receptor signaling pathway; activation of MAPKK activity; nerve growth factor receptor signaling pathway; protein heterooligomerization; apoptosis; MAPKKK cascade; small GTPase mediated signal transduction; Ras protein signal transduction; cytoplasmic sequestering of protein; insulin receptor signaling pathway; innate immune response; gene expression; negative regulation of transcription, DNA-dependent; vascular endothelial growth factor receptor signaling pathway; protein targeting

0.1 mL

395 USD