antibody

Nitrotyrosine (nTyr)

MBS603220

0.1 mg

615 USD

monoclonal

mouse

nonconjugated

6D609

human, mouse, rat, dog, cow

western blot, ELISA, enzyme immunoassay

Antibody

Nitrotyrosine (nTyr)

Nitrotyrosine

Anti -Nitrotyrosine (nTyr)

nitric oxide synthase, inducible; Nitric oxide synthase, inducible; nitric oxide synthase, inducible; NOSII; NOS type II; inducible NOS; inducible NO synthase; inducible nitric oxide synthase; peptidyl-cysteine S-nitrosylase NOS2; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase 2, inducible; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; NOSII; Peptidyl-cysteine S-nitrosylase NOS2

NOS2; NOS2; iNOS; NOS2A; Inducible NOS; iNOS; NOSII

NOS2_BOVIN

Monoclonal

IgG1,k

6D609

Mouse

Canine, Human, Mouse, Rat

Recognizes Nitrotyrosine (nTyr). Species Crossreactivity: human, mouse, rat and canine

Purified. Purified by thiophilic adsorption and size exclusion chromatography.

Supplied as a lyophilized powder from PBS, PEG, sucrose, 0.1% sodium azide. Reconstitute in 1ml sterile ddH2O.

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

ELISA (EL/EIA), Western Blot (WB)

Suitable for use in ELISA and Western Blot. Dilution: ELISA: 0.05ug/ml. Western Blot: 1ug/ml for AP/BCIP/NBT (MTT detection) or 0.1ug/ml for HRPO/ECL detection.

Immunogen: KLH modified with nitrotyrosine

Antibodies; Abs to Nitrotyrosine

The enzymatic conversion of arginine to citrulline by nitric oxide synthase (NO Synthase) leads to the formation of nitric oxide (NO). NO forms peroxynitrite following the reaction with superoxide. Peroxynitrite is a potent oxidant that reacts with a variety of biological molecules, including tyrosine residues in proteins. Tyrosine nitration can inactivate enzymes and receptors that depend on tyrosine residues for their activity. Nitration prevents phosphorylation of tyrosnine residues important for signal transduction.

116003827

NP_001070267.1

NM_001076799.1

Q27995

131,208 Da

Amoebiasis Pathway (167315); Amoebiasis Pathway (167191); Arginine And Proline Metabolism Pathway (84202); Arginine And Proline Metabolism Pathway (323); Calcium Signaling Pathway (84289); Calcium Signaling Pathway (459); Chagas Disease (American Trypanosomiasis) Pathway (147808); Chagas Disease (American Trypanosomiasis) Pathway (147795); Disease Pathway (827627); HIF-1 Signaling Pathway (695207)

Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 . By similarity. Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O. Cofactor: Heme group . By similarity.Binds 1 FAD . By similarity.Binds 1 FMN . By similarity.Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme . By similarity. Enzyme regulation: Regulated by calcium/calmodulin . By similarity. Subunit structure: Homodimer. Binds SLC9A3R1 . By similarity. Sequence similarities: Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain.

0.1 mg

615 USD