antibody

HLA Class 1 Antigen A9,A25,A32

MBS603207

0.1 mL

410 USD

monoclonal

mouse

nonconjugated

[8.L.155]

Antibody

HLA Class 1 Antigen A9,A25,A32

[HLA Class 1 Antigen A9,A25,A32]

[Anti -HLA Class 1 Antigen A9,A25,A32]

Monoclonal

IgM

[8.L.155]

Mouse

Human

Reacts to HLA Class 1 Antigen-A9,A25,A32. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.

Ascites

Supplied as a lyophilized powder in PBS, 1% BSA. Reconstitute to 100ul sterile ddH2O to make a 10X stock solution.

Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

This antibody has not been tested in methodologies other than Microcytotoxicity Test. Potential applications include Flow Cytometry, Cell Typing, Tissue Staining and Chimerism Studies.

Recommended Dilutions: . Cytotoxicity: Add 0.9ml of PBS, 1% BSA to 100ul of10X stock solution. Optimal dilutions to be determined by the researcher

Testing Data

Immunogen: HLA Class 1 Antigen-A9, A25, A32. Grade: Ascites

Antibodies; Abs to HLA

The human leukocyte antigen system (HLA) is the name of the major histocompatibility complex (MHC) in humans. The superlocus contains a large number of genes related to immune system function in humans. This group of genes resides on chromosome 6, and encodes cell-surface antigen-presenting proteins and many other genes. The HLA genes are the human versions of the MHC genes that are found in most vertebrates (and thus are the most studied of the MHC genes). The proteins encoded by certain genes are also known as antigens, as a result of their historic discovery as factors in organ transplantations. The major HLA antigens are essential elements for immune function. Different classes have different functions: HLA class I antigens (A, B & C) present peptides from inside the cell (including viral peptides if present). These peptides are produced from digested proteins that are broken down in the proteasomes. The peptides are generally small polymers, about 9 amino acids in length. Foreign antigens attract killer T-cells (also called CD8 positive- or cytotoxic T-cells) that destroy cells. HLA class II antigens (DP,DM, DOA,DOB,DQ, & DR) present antigens from outside of the cell to T-lymphocytes. These particular antigens stimulate T-helper cells to multiply, and these T-helper cells then stimulate antibody-producing B-cells to produce Antibodies to that specific antigen. Self-antigens are suppressed by suppressor T-cells.

0.1 mL

410 USD