RSPO-3 (R-Spondin 3, Cristin-1) | MyBioSource MBS603109 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

RSPO-3 (R-Spondin 3, Cristin-1)

catalog :

MBS603109

quantity :

0.1 mg

price :

545 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

7H222

reactivity :

human, mouse

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS603109

products type :

Antibody

products full name :

RSPO-3 (R-Spondin 3, Cristin-1)

products short name :

RSPO-3

products name syn :

Anti -RSPO-3 (R-Spondin 3, Cristin-1)

other names :

R-spondin-1 isoform 3; R-spondin-1; R-spondin-1; R-spondin homolog; roof plate-specific spondin-1; R-spondin 1; Roof plate-specific spondin-1

other gene names :

RSPO1; RSPO1; RSPO; CRISTIN3; RP11-566C13.1; hRspo1

uniprot entry name :

RSPO1_HUMAN

clonality :

Monoclonal

isotype :

IgG2a

clone :

7H222

host :

Rat

reactivity :

Mouse

specificity :

Recognizes mouse RSPO-3. No crossreactivity with recombinant mouse RSPO-1, RSPO-4, recombinant human RSPO-2 or RSPO-3.

purity :

Affinity Purified. Purified by Protein G affinity chromatography.

form :

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS, pH 7.4.

storage stability :

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

tested application :

ELISA (EL/EIA), Western Blot (WB)

app notes :

Suitable for use in Direct ELISA and Western Blot. Dilution: Western Blot: 1ug/ml. Optimal dilutions to be determined by researcher.

other info1 :

Immunogen: Recombinant corresponding to aa21-209 of mouse RSPO-3 expressed in E. coli (Accession #BAC36296).

other info2 :

Hybridoma: Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, E. coli-derived, recombinant mouse RSPO-3.

products categories :

Antibodies; Abs to Proteins

products description :

RSPO-3 (R-spondin 3; also Cristin-1), is a 256aa secreted R-Spondin family member which binds LRP-6 and Fzd8 and regulates b-catenin signaling. R-Spondins contain two adjacent cysteine-rich furin-like domains at their amino-termini, followed by a thrombospondin (TSP-1) motif and a variable length basic C-terminal region. Two RSPO-3 splice isoforms that differ in their C-termini have been reported. Mouse RSPO-3 shares 93% and 97% aa identity with human and rat RSPO-3, respectively, over the sequence used as an immunogen.

ncbi gi num :

339276103

ncbi acc num :

NP_001229839.1

ncbi gb acc num :

NM_001242910.1

uniprot acc num :

Q2MKA7

ncbi mol weight :

28,959 Da

ncbi pathways :

Wnt Signaling Network Pathway (137962)

ncbi summary :

This gene is a member of the R-spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. [provided by RefSeq, Jul 2008]

uniprot summary :

R-spondin-1: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC). This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Cell adhesion; Secreted, signal peptide; Secreted; Cell development/differentiation. Chromosomal Location of Human Ortholog: 1p34.3. Cellular Component: extracellular space; extracellular region; nucleus. Molecular Function: heparin binding; protein binding; G-protein-coupled receptor binding; receptor binding. Biological Process: regulation of gene expression; regulation of receptor internalization; Wnt receptor signaling pathway through beta-catenin; positive regulation of protein amino acid phosphorylation; male meiosis; positive regulation of Wnt receptor signaling pathway. Disease: Palmoplantar Hyperkeratosis With Squamous Cell Carcinoma Of Skin And 46,xx Sex Reversal

size1 :

0.1 mg

price1 :

545 USD

more info or order :

MyBioSource product webpage