antibody

HLA Class 1 Antigen Bw4 (FITC)

MBS603035

0.25 mL

410 USD

monoclonal

mouse

FITC

[0.L.6]

immunocytochemistry, flow cytometry, FACS

Antibody

HLA Class 1 Antigen Bw4 (FITC)

[HLA Class 1 Antigen Bw4]

[Anti -HLA Class 1 Antigen Bw4 (FITC)]

[HLA class I histocompatibility antigen, Cw-1 alpha chain; HLA class I histocompatibility antigen, Cw-3 alpha chain; HLA class I histocompatibility antigen, Cw-1 alpha chain; MHC class I antigen heavy chain HLA-C; human leukocyte antigen-C alpha chain; major histocompatibility antigen HLA-C; HLA class I histocompatibility antigen, C alpha chain; major histocompatibility complex, class I, C; MHC class I antigen Cw*3]

[HLA-C; HLA-C; HLC-C; D6S204; PSORS1; HLA-JY3; HLAC]

1C03_HUMAN

Monoclonal

IgG2a

[0.L.6]

Mouse

Human

Recognizes HLA Class 1 Antigen-Bw4 when used at a dilution of 1:10 when using the microcytoxicity test under standard NIH conditions.

Highly Purified. Purified by Ion Exchange chromatography.

Supplied as a liquid in PBS, 0.1% sodium azide, 1% BSA. Labeled with fluorescein isothiocyanate (FITC). FITC

0.1 mg/ml-O.5mg/ml

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Flow Cytometry (FC/FACS), Immunofluorescence (IF)

Suitable for use in Flow Cytometry, Cell Typing, Tissue Staining and Chimerism Studies. Dilution: Flow Cytometry: 10ul/test

Protein Ratio: 4.0-8.0

Antibodies; Abs to HLA

Antibodies can also be used for the detection of HLA antigens on the lymphocytes in whole blood samples and cells isolated from blood or lymphoid tissue. The HLA Class 1 conjugated monoclonal antibodies can be used for the detection of specific cell surface antigens. Intended Use. These reagents are used for direct immunofluorescence staining of specific cell surface antigens with analysis by flow cytometry . Antibodies can also be used for the detection of HLA antigens on the lymphocytes in whole blood samples and cells isolated from blood or lymphoid tissue. Summary and Explanation. The HLA Class 1 conjugated monoclonal antibodies can be used for the detection of specific cell surface antigens. Principle. Conjugated monoclonal antibodies(Mabs) detect cells bearing the cell surface antigens specific to the antibody. Cells (whole blood or isolatea cells) are stained with the conjugated Mab, red blood cells are lysed and white blood cells are fixed with formaldehyde. Flow cytometric anlysis is performed on the target cells. Identification. HLA Antigen Specific FITC Antibody Concentration: 0.1 mg/ml-O.5mg/ml Total volume: 250ul sufficient for 25 tests. F:P Ratio: 4.0-8.0. Note: All blood products should be treated as potentially infectious. Source material from which this product was derived was found negative when tested in accordance with current FDA required tests. No known test methods can offer assurance that products denved from known human blood will not trasmit infectious agents. Treatment Required for Use. 1. To avoid volume loss, centrifuge conjugated Mab vial for a few seconds in a microcentrifuge before opening (liquid may accumulate in cap during shipment). 2.vial is susceptible to volume loss if left uncovered. Cover vial immediately after use. Instability Indications. Do not use the reagent if precipitate is observed. Specimen Colledion and Preparation. 1. The blood specimen should be collected in an ACD (Acid Citrate Dextrose) Vacutainer blood collection tube and analyzed within three days. However, EDTA (K3) or Sodium Heparin may also be used. 2. Sterile blood samples should be stored horizontally at room temperature (20°C) and analyzed within 3 days of collection. Procedure. A. Materials Provided. 1. FITC Conjugated Monoclonal Antibody. Warning: These reagents may contain sodium azide in the range of 0.1-1 % which under acidic conditions yields hydrazonic acid, an extremelY toxic compound. Reagents containing sodium azide snould be diluted in running water prior to being discarded. These conditions are recommended to avoid deposits in plumbing where explosive conditions may develop. See European Directive 67/548/EEC (Xn Harmful). B. Materials Required, But Not Provided. 1. Red Blood Cell lysing solution. 2. Streptavidin-PE. 3. Phosphate Buffered Saline (PBS) or equivalent. 4. Fixing solution: PBS with 0.5% formaldehyde (add 1.35ml 37% formaldehyde to 100ml PBS). Warning: Formaldehyde is toxic and allergenic, and is a suspected carcinogen. Formaldehyde is listed as a carcinogen in California. Avoid contact with eyes, skin and clothing. Diredions for Use. A. Whole Blood Staining Method To avoid volume loss, centrifuge vial for a few seconds in a microcentrifuge before use (liquid may accumulate in cap during shipment). 1. Pipette 1 OOul of whole blood sample into a 12x75mm tube. 2. Pipette 10ul of H6098-61 A into the tube and mix well with gentle vortex. Caution: Susceptible to volume loss if vial is left uncovered. Cover vial immediately ater use. 3. Incubate in the dark at 4°C for 30 minutes while gently shaking. 4. Lyse Red Blood Cells. 5. Centrifuge samples at 300 x g for 5 minutes. Discard the supernatant. 6. Resuspend pellet in 2ml of PBS, mix, and centrifuge at 300 x g for 5 minutes. Discard the supernatant. 7. For FITC conjugated Mab! go to step #9. 8. Repeat steps 5 and 6 twice. 9. Resuspend the pellet in 0.5 ml of fixing solution. The cells are ready for immediate flow cytometry analysis, or can be stored in the. dark at 4°C for up to 24 hours before being analyzed. B. Isolated Cell Staining Method. 1. Isolate leukocytes from heparinized blood or tissue samples by Ficoll-Hypaque density gradient centrifugation. 2. Wash the cells twice with McCoy's and centrifuge at 300 x g for 5 minutes. 3. Resuspend the cells in McCoy's and adjust the concentration to 1 xl Oe6 cell/ml. 4. Incubate 100ui (lOe5 cells) with lOul of H6098-61A in the dark at 4°C for 20 minutes. 5. Wash the cells twice with 2ml PBS at 300 x g for 5 minutes. 6. For FITC conjugated Mab, go to step #8. 7. Repeat step 5. 8. Resuspend the pellet in O.5ml of fixing solution. The cells are ready for immediate flow cytometry analysis, or can be stored in the dark at 4°C for 24 hours before being analyzed. Expeded Values. Each laboratory should establish the normal ranges of positive cells and negative cells under its own testing conditions for each batch of samples. FITC conjugated monoclonal antiboaies should show a FL 1 channel shift where it reacts with tfle positive cells when compared to the negative cells. Biotin conjugated monoclonal antibodies combined with the usage of streptavidin-PE should show a FL2 channel shift wnen reacting with the positive cells. Different FITC or Biotin conjugated monoclonal antibodies may have different ranges of channel shift for the positive reactions. In addition, individual lots of FITC or Biotin conjugated monoclonal antibodies may have different channel shifts for the positive reactions. Specific Performance Charaderistics. Normal lymphocytes should all express HLA-Class I antigens. Therefore, lymphocytes should be selected by proper gates on FSC versus SSC dot plots for the studies of HLA-Class I antigens. B cells express both Class I and Class II antigen. T cells do not express class II antigens. Therefore, when Class II antigens are studied using the biotinylated monoclonal antibodies, selection of B cells using CD20-FITC with streptavidin-PE is the preferred method.

52630342

NP_002108.4

NM_002117.5

P04222

40,861 Da

Adaptive Immune System Pathway (366160); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway (366163); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Antigen Processing-Cross Presentation Pathway (477122); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Cell Adhesion Molecules (CAMs) Pathway (83069)

HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Over one hundred HLA-C alleles have been described [provided by RefSeq, Jul 2008]

HLAC iso7: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family. Protein type: Receptor, misc.; Membrane protein, integral. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: Golgi membrane; Golgi apparatus; phagocytic vesicle membrane; cell surface; early endosome membrane; integral to plasma membrane; endoplasmic reticulum; extracellular region; plasma membrane; MHC class I protein complex. Molecular Function: peptide antigen binding; TAP binding; receptor binding. Biological Process: regulation of immune response; antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent; viral reproduction; positive regulation of T cell mediated cytotoxicity; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; innate immune response; immune response

0.25 mL

410 USD