product summary
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company name :
MyBioSource
product type :
antibody
product name :
a-Actin, Skeletal Muscle
catalog :
MBS602996
quantity :
0.5 mL
price :
450 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
0.N.4
reactivity :
human, rat, chicken, cow
application :
immunohistochemistry, immunocytochemistry
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS602996
products type :
Antibody
products full name :
a-Actin, Skeletal Muscle
products short name :
a-Actin, Skeletal Muscle
products name syn :
Anti -a-Actin, Skeletal Muscle
other names :
actin, alpha skeletal muscle; Actin, alpha skeletal muscle; actin, alpha skeletal muscle; a-actin; alpha-actin-1; actin, alpha 1, skeletal muscle; Alpha-actin-1
other gene names :
ACTA1; ACTA1; ACTA
uniprot entry name :
ACTS_CHICK
clonality :
Monoclonal
isotype :
IgM,k
clone :
0.N.4
host :
Mouse
reactivity :
Bovine, Frog, Guinea Pig, Human, Rabbit, Rat, Sheep
specificity :
Recognizes human alpha-skeletal and alpha-cardiac (sarcomeric) actins as well as neoplasms derived from normal tissues (i.e. rhabdomyosarcomas). Shows no crossreaction with smooth muscle actin. Species Crossreactivity: bovine, rabbit, sheep, rat, guinea pig and frog.
purity :
Purified. Purified by ammonium sulfate precipitation.
form :
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide, before the addition of glycerol to 40%. Also available without BSA and azide. See A0760-24X.
storage stability :
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
tested application :
Immunohistochemistry (IHC), Immunofluorescence (IF)
app notes :
Suitable for use in Immunofluorescence and Immunohistochemistry. Dilution: Immunohistochemistry (formalin/paraffin sections): 1:200 for 20 min at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues.
other info1 :
Immunogen: N-terminal decapeptide of alpha skeletal muscle isoform of actin; acetylated at the N-terminus. Cellular Localization: Cytoplasmic. Epitope: N-terminal decapeptide.
other info2 :
Positive Control: Skeletal or Cardiac muscle, or Rhabdomyosarcoma
products categories :
Antibodies; Abs to Actin Related Proteins
ncbi gi num :
71894831
ncbi acc num :
NP_001026234.1
ncbi gb acc num :
NM_001031063.1
uniprot acc num :
P68139
ncbi mol weight :
42,051 Da
uniprot summary :
Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Subunit structure: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Subcellular location: Cytoplasm cytoskeleton. Post-translational modification: Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization . By similarity. Miscellaneous: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. Sequence similarities: Belongs to the actin family.
size1 :
0.5 mL
price1 :
450 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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