product summary
Loading...
company name :
MyBioSource
product type :
antibody
product name :
Anti - Histones
catalog :
MBS602894
quantity :
0.1 mg
price :
750 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
[2Q2205]
application :
western blot, ELISA, enzyme immunoassay, other
more info or order :
image
image 1 :
Jurkat cell lysate was probed with MBS602894 at 0.5ug/ml. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Arrow indicates Histone H1 + core proteins (~14-33kD).
product information
catalog number :
MBS602894
products type :
Antibody
products full name :
Anti - Histones
products short name :
[Histones]
products name syn :
[Histones]
clonality :
Monoclonal
isotype :
IgG2a,k
clone :
[2Q2205]
host :
Mouse
reactivity :
Bovine and Hamster.
specificity :
Recognizes human Histones, H1 and core proteins H2a, H2b, H3, H4.
purity :
Purified by Protein G affinity chromatography.
form :
Supplied as a liquid in Tris-Glycine,pH 7.4, 0.15M sodium chloride 0.05% sodium azide.
concentration :
1 mg/ml
storage stability :
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
tested application :
Suitable for use in ELISA (EIA) and Western Blot (WB). Other applications have not been tested.
app notes :
Western Blot: 0.5 ug/ml detects Histone H1 and core proteins in 10 ug of Jurkat cell lysate. Optimal dilutions to be determined by the researcher.
image1 heading :
Testing Data
other info1 :
Immunogen: Histones purified from calf thymus. MW: 15-33kD.
other info2 :
Positive Control: Jurkat cell lysate.
products categories :
Abs to Histones
products description :
Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H1 is located in the nuclear and is necessary for the condensation of nucleosome chains into higher order structures. Histone H1 produces a speckled staining pattern in the nucleus of normal and malignant cells.
products references :
1. Federation Proceedings (1984). 43:2063. 2. Mizoguchi, E., et al., Antineutrophil Cytoplamic Antibodies in T-Cell Receptor •-Deficient Mice with Chronic Colitis. Gastroenterology (1997) 113:1828-1835. 3. Feng, H., et al., CUL-2 is required for the G1-to-S-phase transition and mitotic chromosome condensation in Caenorhabditis elegans. Nature Cell Biology (1999) 1:486- 492. 4. Brimmell, M., et al., High level expression of differentially localized BAG-1 isoforms in some oestrogen receptor-positive human breast cancers. British Journal of Cancer (1999) 81:1042-1051.
ncbi acc num :
NM_005321.2
size1 :
0.1 mg
price1 :
750 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!