antibody

Vitellogenin (killifish)

MBS602800

0.1 mL

615 USD

monoclonal

mouse

nonconjugated

[6D836]

western blot, ELISA, enzyme immunoassay

Antibody

Vitellogenin (killifish)

[Vitellogenin]

[Anti -Vitellogenin (killifish)]

[vitellogenin; Vitellogenin; vitellogenin; vitellogenin<]

[Vg; Vg; GB13999]

VIT_APIME

Monoclonal

IgG1, k

[6D836]

Mouse

Recognizes vitellogenin from killifish (Fundulus heteroclitus) and white perch (Morone americanus). Cossreacts with vitellogenin from a variety of different species including common carp (Cyprinus carpio), wrasse (Ctenolabrus rupestris), tilapia (Oreochromis niloticus), halibut (Hippoglossus hippoglossus) and sheepshead minnow (Cyprinodon variegatus). Does not react with vitellogenin from bowfin (Amia calva) or with egg yolk from swordfish (Xiphias gladius).

Affinity Purified. Purified by immunoaffinity chromatography.

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

ELISA (EL/EIA), Western Blot (WB)

Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:100-1:1000. Western Blot: 1:100-1:500

ELISA (EIA)

Western Blot (WB)

Immunogen: Vitellogenin from plasma of 17beta-estradiol treated killifish (Fundulus heteroclitus), white perch (Morone americanus), Bowfish (Amia Calva). Egg yolk from swordfish (Xiphias gladius).

Antibodies; Abs to Fish

Vitellogenin (Vg) is an egg yolk precursor protein expressed in female fish, dormant in male fish and female insects, but in the presence of estrogenic endocrine disrupting chemicals (EDCs), males can express the Vg gene in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDCs.

58585104

NP_001011578.1

NM_001011578.1

Q868N5

201,048 Da

Function: Precursor of the egg-yolk proteins that are sources of nutrients during embryonic development. By similarity. Involved in the differentiation of honeybee larvae into queens. Ref.2. Subcellular location: Secreted. Tissue specificity: Accumulates in the hemolymph. Represents up to 70% of the queen's hemolymph proteins. During the first week of the worker adult life, when it becomes a nurse bee and performs brood-rearing tasks, the vitellogenin titer increases and may account for up to 40% of the total hemolymph proteins. Ref.1. Developmental stage: In queens, first expressed in mid-late pupal stage and a high production is maintained throughout adult life. In workers, observed in late pupal stage and expressed at low levels in adults. Levels can increase in some young workers if an egg-laying queen is missing to the colony. Also detected in drones, but in freshly molted adults and not in pupae. Ref.1. Induction: By MRJP1 during the differentiation of honeybee larvae into queens. Ref.2. Sequence similarities: Contains 1 vitellogenin domain.Contains 1 VWFD domain.

0.1 mL

615 USD