antibody

Ephrin B, pan

MBS602769

0.05 mg

540 USD

monoclonal

mouse

nonconjugated

6A72

western blot, ELISA, immunoprecipitation, enzyme immunoassay

Antibody

Ephrin B, pan

Ephrin B, pan

Anti -Ephrin B, pan

epoxide hydrolase EphB

Monoclonal

IgG1,k

6A72

Mouse

Chicken, Human

Recognizes over-expressed and endogenous ephrin-B, as well as phosphorylated ephrin-B. On Western Blots it identifies a band at ~50-60kD. Species crossreactivity

Affinity Purified. Purified by Protein A chromatography

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide

0.5mg/ml

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)

Suitable for use in Western Blot, Immunoprecipitation and ELISA. Recommended Dilutions: Optimal dilutions to be determined by the researcher.

Immunogen: Synthetic peptide corresponding to the ephrin-B sequence.

Positive Control: Human 293T and MCF10A lysates, chicken cell lysates

Antibodies; Abs to Protein Kinases

Ephrins are a family of proteins that bind to the tyrosine kinase Eph receptor. Their function is still under investigation, but they are suspected to play an important role in morphogenesis. The ephrins can be grouped into two classes: those that are tethered to the membrane by a GPI linkage, called ephrin-A proteins (which bind to EphA receptors), and those with a membrane-spanning region, called ephrin-B proteins (which bind to EphB receptors). Ephrins are expressed in the embryo in various tissues in the forming mesoderm, endoderm and nervous system in all of the vertebrate species in which they have been identified. Ephrin-B3, also known as NLERK-2, Elk-L3, EFL-6, ELF-3 and LERK-8 is a member of the ephrin-B protein family. Ephrin-B3 binds EphA4, EphB1, EphB2 and EphB33,. Ephrin-B1 is one of the transmembrane ligands for the EphB2 receptor. In the embryo, EphB2 and ephrin-B1 participate in neuronal axon guidance, neural crest cell migration, the formation of blood vessels and the development of facial structures and the inner ear. EphB2 and ephrin-B1 can both signal through their cytoplasmic domains and become phosphorylated when bound to each other. Tyrosine phosphorylation regulates EphB2 signaling and likely also ephrin-B1 signaling.

31793130

NP_855623.1

NC_002945.3

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

0.05 mg

540 USD