Carbonic Anhydrase XII | MyBioSource MBS602759 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Carbonic Anhydrase XII

catalog :

MBS602759

quantity :

0.1 mg

price :

545 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7H81

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS602759

products type :

Antibody

products full name :

Carbonic Anhydrase XII

products short name :

Carbonic Anhydrase XII

products name syn :

Anti -Carbonic Anhydrase XII

other names :

Carbonic anhydrase XII; Carbonic anhydrase 12; carbonic anhydrase 12; CA-XII; OTTHUMP00000163786; OTTHUMP00000163787; carbonic dehydratase; carbonate dehydratase XII; tumor antigen HOM-RCC-3.1.3; carbonic anhydrase XII; Carbonate dehydratase XII; Carbonic anhydrase XII; CA-XII; Tumor antigen HOM-RCC-3.1.3

other gene names :

CA12; CA12; CAXII; FLJ20151; HsT18816

uniprot entry name :

CAH12_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

7H81

host :

Mouse

reactivity :

Human

specificity :

Recognizes human Carbonic Anhydrase XII (CA12). Does not crossreact with recombinant human CA1, -2, -3, -4, -5, -7, -8, -10, -11, -13 or -14.

purity :

Affinity Purified. Purified by Protein G affinity chromatography.

form :

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

storage stability :

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

tested application :

ELISA (EL/EIA), Western Blot (WB)

app notes :

Suitable for use in Western Blot and ELISA. Dilution: Western Blot: 1-2ug/ml. Using a colormetric detection system, the detection limit is ~5ng/lane under non-reducing and reducing conditions. Direct ELISA: 0.5-1ug/ml. The detection limit is ~10ng/well.

other info1 :

Immunogen: Recombinant corresponding to aa25-291of human Carbonic Anhydrase XII expressed in NSO cells (NP_001209).

products categories :

Antibodies; Abs to Enzymes

products description :

Carbonic anhydrase XII (CA12) is a type I membrane enzyme highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. It is over-expressed in some renal cell cancers and in glaucoma. Two alternatively spliced forms exist, which either contain or lack an 11 amino acid segment just before the transmembrane domain. The amino acid sequence of human CA12 is 97%, 84%, 82%, 81%, and 67% identical to that of chimpanzee, canine, mouse, rat and chicken.

ncbi gi num :

18645129

uniprot acc num :

O43570

ncbi mol weight :

39,451 Da

ncbi pathways :

Nitrogen Metabolism Pathway (83025); Nitrogen Metabolism Pathway (416)

ncbi summary :

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq]

uniprot summary :

CA12: Reversible hydration of carbon dioxide. Homodimer. Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis. Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA), benzenesulfonamide and derivatives (4-carboxyethylbenzene-sulfonamide, 4- carboxyethylbenzene-sulfonamide ethyl ester, 4-(acetyl-2- aminoethyl)benzene-sulfonamide, 4-aminoethylbenzene-sulfonamide) and Foscarnet (phosphonoformate trisodium salt). Belongs to the alpha-carbonic anhydrase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Energy Metabolism - nitrogen; EC 4.2.1.1; Lyase. Chromosomal Location of Human Ortholog: 15q22. Cellular Component: plasma membrane; integral to membrane. Molecular Function: carbonate dehydratase activity; zinc ion binding. Biological Process: bicarbonate transport; one-carbon compound metabolic process; chloride ion homeostasis. Disease: Hyperchlorhidrosis, Isolated

size1 :

0.1 mg

price1 :

545 USD

more info or order :

MyBioSource product webpage