antibody

Bmi-1, aa1-202 (RNF51, MGC12685, BUP, Polycomb Group RING Finger Protein 4)

MBS602584

0.05 mg

520 USD

monoclonal

mouse

nonconjugated

[1.T.21]

western clawed frog, human, mouse, rat

Antibody

Bmi-1, aa1-202 (RNF51, MGC12685, BUP, Polycomb Group RING Finger Protein 4)

[Bmi-1, aa1-202]

[Anti -Bmi-1, aa1-202 (RNF51, MGC12685, BUP, Polycomb Group RING Finger Protein 4)]

[BMI1 polycomb ring finger oncogene; BMI1 polycomb ring finger oncogene; BMI1 polycomb ring finger oncogene]

[bmi1; bmi-1; pcgf4; rnf51; xbmi-1]

Monoclonal

IgG1

[1.T.21]

Mouse

Human, Mouse, Rabbit, Rat

Recognizes mouse Bmi-1 (triplet), Mr 40-44kD.Species Crossreactivity: human, rat and rabbit.

Affinity Purified. Purified by Protein G affinity chromatography.

Supplied as a liquid in 0.1M Tris-Glycine, 0.15M sodium chloride, pH 7.4, 0.05% sodium azide.

1mg/ml

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC). Other applications have not been tested.

Recommended Dilution: Western Blot: 0.2-2ug/ml. Detects Bmi-1 in RIPA lysates from U2OS cells. U2OS cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with MBS602584. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Immunoprecipitation: Reported to immunoprecipitate Bmi-1 from mouse embryo protein extracts. Immunocytochemistry: Reported to immunostain U2OS cells fixed with 2% formaldehyde. Optimal dilutions to be determined by the researcher. Recommended Positive Control: U2OS whole cell lysate, K-562 nuclear extract, 0-4 hour embryos or 12-24 hour embryos.

Western Blot (WB)

Western Blot (WB)

Control No.: C15020460. Acession No.: NP_005171. Immunogen: Recombinant Bmi-1 protein corresponding to aa1-202 of mouse Bmi-1 (NP_005171).

Antibodies; Abs to Transcription Factors, Zinc (Ring) Finger

Bmi1 is a member of the polycomb group (PcG) proteins. PcG proteins form multiprotein complexes that function as transcriptional repressors. Several lines of evidence implicate Bmi1 in tumorigenesis. Firstly, Bmi1 cooperates with c-Myc in the generation of lymphomas in double transgenic mice. Secondly, Bmi1 blocks senescence and immortalizes mouse embryo fibroblasts, although not human fibroblasts, and in combination with an activated H-ras gene leads to neoplastic transformation. Thirdly, the BMI1 gene is amplified in certain mantle cell lymphomas and is overexpressed in a subset of non-small cell lung cancer, colorectal carcinomas, multiple myelomas and medulloblastomas, but not glioblastomas. Fourthly, the transformation of human hematopoietic progenitor cells by the oncogenic fusion protein E2a-Pbx1 requires Bmi1. Based on a list of genes differentially expressed in a mouse model of metastatic prostate cancer on a wild-type and bmi1-deficient genetic background, a gene signature was recently identified that may predict response to therapy and survival in multiple types of cancer.

62858263

NP_001015995.1

NM_001015995.2

0.05 mg

520 USD

0.1 mg

875