antibody

CD257

MBS602561

0.1 mg

515 USD

monoclonal

mouse

nonconjugated

[7H149]

ELISA, flow cytometry, FACS, enzyme immunoassay

Antibody

CD257

[CD257]

[Anti -CD257]

[cytochrome oxidase subunit I]

Monoclonal

IgG1,k

[7H149]

Mouse

Human

Binds to CD257 in EIA and blocks binding of recombinant in Flow cytometry, and blocks binding of recombinant CD257-muCD8 to receptors on Raji cell surface in Flow cytometry.

Affinity Purified. Purified by Protein A affinity chromatography.

Supplied as a liquid in FBS, 50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate.

1 mg/ml

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

ELISA (EL/EIA), Flow Cytometry (FC/FACS)

Suitable for use in Flow Cytometry and ELISA., Recommended DilutionOptimal dilutions to be determined by the researcher.

Immunogen: Recombinant soluble human CD257. Functional Application: Antibody blocks binding of Recombinant CD257(BAFF) to Raji cell surface.

Antibodies; Abs to CD Markers

Human CD257 (B cell activating factor, BAFF,TALL-1, Blys, THANK) and CD256 (APRIL, a proliferation inducing ligand) are both type II molecules belonging to the TNF superfamily (TNFSFL #13b and 13 respectively). They are expressed by non-B cells, and are down regulated by mitogenic stimulation(2). BAFF and APRIL bind to at least two receptors: CD267 (TACI, transmembrane activator and CAML-interactor) and CD269 (BCMA, B cell maturation antigen), both of which are restricted to B cells(3,4). Ligation of these receptors with recombinant BAFF dramatically increases IgM production by peripheral blood B cells(1). A third receptor CD268 (BAFFR) is specific for BAFF has also been described(5). BAFF and BAFFR knockout mice have a reduced numbers of mature B cells in the periphery, however TACI and BCMA knockouts do not share this phenotype, suggesting that BAFF-R may the primary receptor for BAFF in mice(8,9,10). Cell surface BAFF can be proteolytically cleaved to form a soluble trimeric molecule(2). Levels of soluble BAFF correspond with levels of autoantibodies in Sjogren's Syndrome(11).

329757342

AEC04786.1

Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.

0.1 mg

515 USD