catalog number :
MBS602291
products full name :
Microcystin LR
products short name :
Microcystin LR
products name syn :
Anti -Microcystin LR
other names :
Microcystin LR degradation protein MlrC
specificity :
Recognizes all 4-Arg microcystins.
purity :
Affinity Purified. Purified by Protein G affinity chromatography.
form :
Supplied as a lyophilized powder. Reconstitute with 200ul sterile PBS, sodium azide.
concentration :
~1mg/ml (after reconstitution)
storage stability :
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS, sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
tested application :
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
app notes :
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Dilution: ELISA: 1:10,000-1:100,000. Immunohistochemistry: Paraffin sections. Concentrations can be measured in the range of ~0.006ug/L - 0.3ug/L. The ELISA sensitivity is about 1000 times higher compared to UV detection.
other info1 :
Immunogen: Microcystin-LR linked via N-methyl-dehydroalanine to cationized ovalbumin.
products categories :
Antibodies; Abs to Peptides
products description :
With a direct competitive enzyme-linked immunosorbent assay (ELISA) using this antibody, IC50 values for microcystin-LR of 0.06ug/l have been obtained. The provisional guideline value proposed by the WHO is 1ug/l for drinking water. The detection limit for microcystin-LR is 0.006ug/l. All microcystin variants containing an arginine at position 4 show similar IC50 values and detection limits, whereas other microcystins, such as microcystin-LA are not recognized. The affinity constant for this product was determined to be at least 7 x 10e10 l/mol. The antibody was tested for its robustness against interferences (humid acids, pH, salt content, surfactants or organic solvents) and was found to be very stable. Microcystin-LR spiked water samples in the concentration range between 0.01 and 0.1ug/l were measured and a mean recovery of 99.9 ± 16.4% was found. The antibody is well suited for the sensitive analysis of microcystins in drinking as well as surface water.
ncbi acc num :
AEL26184.1