antibody

JAK1 (Janus Kinase 1)

MBS602240

0.1 ml

385 USD

monoclonal

rabbit

nonconjugated

7H32

human, mouse, rat, zebrafish

western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry, FACS, enzyme immunoassay

Antibody

JAK1 (Janus Kinase 1)

JAK1

Anti -JAK1 (Janus Kinase 1)

jak1; Tyrosine-protein kinase JAK1; tyrosine-protein kinase JAK1; jak-1; jak1 kinase; etID309794.6; Janus kinase 1; Janus kinase 1

jak1; jak1; jak1

JAK1_DANRE

Monoclonal

IgG

7H32

Host: Rabbit; Source: Human

Human, Mouse, Rat

Detects endogenous levels of total Jak1 protein. It does not cross react with other Jak family members. Species sequence homology

Supernatant. Supernatant

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Suitable for use in ELISA, Western Blot, Immunoprecipitation, Flow Cytometry and Immunohistochemistry. Dilution: Western blot 1:1000. Immunoprecipitation 1:50. Immunohistochemistry (Paraffin) 1:100

Immunogen: Synthetic peptide (KLH-coupled) corresponding to residues surrounding Ile-800 of Jak1.

Antibodies; Abs to Protein Kinases

The Janus family of tyrosine kinases, consisting of Jak1, Jak2, Jak3 and Tyk2, associates with a variety of cytokine receptors and are activated by ligand binding to these receptors (1). Upon ligand binding to cytokine receptors, Jaks autophosphorylate and phosphorylate their associated receptors, providing multiple binding sites for signaling proteins containing SH2 or other phosphotyrosine- binding domains, including Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (FAK) (5)

226237498

O12990

132,481 Da

EGFR1 Signaling Pathway 198963!!IL-2 Signaling Pathway 198953!!Jak-STAT Signaling Pathway 83643!!Jak-STAT Signaling Pathway 488

Function: Tyrosine kinase of the non-receptor type. Appears to be required in early development for specific cell migrations (epiboly), expression of homeobox protein goosecoid and formation of anterior structures. Ref.1. Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Subcellular location: Endomembrane system; Peripheral membrane protein. By similarity. Note: Wholly intracellular, possibly membrane associated. Developmental stage: Present in the unfertilized egg through to the blastula stage where it is distributed uniformly. Levels drop rapidly at four hours development, remain very low until 10 hours, then gradually increase from 12 hours with a rapid increase at 48 hours. At 48 hours it is concentrated in the region of the gill arches. Also present in the adult. Ref.1. Domain: Possesses two phosphotransferase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for domain 1. Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.Contains 1 FERM domain.Contains 1 protein kinase domain.Contains 1 SH2 domain.

0.1 ml

385 USD