product summary
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company name :
MyBioSource
product type :
antibody
product name :
Mps1, NT
catalog :
MBS602045
quantity :
0.2 mg
price :
645 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
[5H8]
application :
western blot, immunocytochemistry
more info or order :
image
image 1 :
HeLa total cell lysate was resolved by electrophoresis, transferred to PVDF and probed with MBS602045 (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Mps1 (97kD).
product information
catalog number :
MBS602045
products type :
Antibody
products full name :
Mps1, NT
products short name :
[Mps1]
products name syn :
[Anti -Mps1, NT]
other names :
[MPS1; Serine/threonine-protein kinase MPS1; Monopolar spindle protein 1; Regulatory cell proliferation kinase 1]
other gene names :
[MPS1; RPK1]
uniprot entry name :
MPS1_YEAST
clonality :
Monoclonal
isotype :
IgG1
clone :
[5H8]
host :
Mouse
reactivity :
Human
specificity :
Recognizes human Mps1, Mr ~97kD. Does not react with mouse.
purity :
Affinity Purified. Purified by Protein G affinity chromatography.
form :
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
concentration :
1mg/ml
storage stability :
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
tested application :
Western Blot (WB), Immunocytochemistry (ICC)
app notes :
Suitable for use in Western Blot, Immunocytochemistry and Kinase assays. Dilution: . Western Blot: 0.5-2ug/ml of this lot detected Mps1 HeLa total cell extract. Kinase Assay: Has been reported by an independent laboratory to immunoprecipitate Mps1 from HeLa total cell extract. The immunoprecipitates were then used in a radioactive kinase assay. Immunocytochemistry: Has been reported by an independent laboratory to show positive immunostaining for Mps1 in U2OS or HeLa cells permeabilized with 0.5% Triton-X-100 and fixed with methanol or paraformaldehyde.
image1 heading :
Testing Data
other info1 :
Immunogen: His-tagged fusion protein corresponding to aa3-856 of human Mps1.
products categories :
Antibodies; Abs to Protein Kinases
ncbi gi num :
1431004
uniprot acc num :
P54199
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: Involved in the regulation of the onset of mitosis. Involved in a pathway that coordinates cell proliferation and differentiation. Implicated in spindle pole body (SPD) duplication. Dual specificity kinase that can phosphorylate serine, threonine and tyrosine residues. Phosphorylates the SPC29 and SPC110 spindle pole body components. Ref.6 Ref.7. Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Post-translational modification: Autophosphorylated. Sequence similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.Contains 1 protein kinase domain.
size1 :
0.2 mg
price1 :
645 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
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