antibody

Akt1, PH Domain, Serine-Threonine Phosphorylation (Rac PKa, PKBa)

MBS601744

0.1 mg

570 USD

monoclonal

mouse

nonconjugated

1.T.1

human, mouse, rat

western blot, immunocytochemistry, immunoprecipitation, flow cytometry, FACS

Antibody

Akt1, PH Domain, Serine-Threonine Phosphorylation (Rac PKa, PKBa)

Akt1, PH Domain, Serine-Threonine Phosphorylation

Anti -Akt1, PH Domain, Serine-Threonine Phosphorylation (Rac PKa, PKBa)

AKT1; RAC-alpha serine/threonine-protein kinase; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; protein kinase B; proto-oncogene c-Akt; rac protein kinase alpha; v-akt murine thymoma viral oncogene homolog 1; Protein kinase B; PKB; Proto-oncogene c-Akt; RAC-PK-alpha

AKT1; AKT1; AKT; PKB; RAC; PRKBA; MGC99656; PKB-ALPHA; RAC-ALPHA; PKB; RAC

AKT1_HUMAN

Monoclonal

IgG

1.T.1

Mouse

Human, Mouse, Rat

Recognizes human Akt1/PKBa > Akt2/PKBb (30%) > Akt3/PKBg (weak to none) based on Western blot analysis using 100ng recombinant fusion protein for each isoform.

Affinity Purified. Purified by Protein G affinity chromatography.

Supplied as a liquid in PBS, 0.05% sodium azide.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)

Suitable for use in Flow Cytometry, Western Blot, Immunocytochemistry, Immunoprecipitation Kinase and Immunoprecipitation. Dilution: Western Blot Analysis: 0.1-1ug/ml detects Akt (60kD) in RIPA lysates from A431 cells. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A1125-04 (0.5 and 1.0ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4ug immunoprecipitates Akt from 500ug of L6 RIPA lysate. Immunoprecipitation Kinase: 4ug immunoprecipitates active Akt from 700ug of L6 cells stimulated with 15nM IGF-1 for 2-5 min.

Immunogen: GST-fusion protein corresponding to residues 1-149 of human Akt1

Antibodies; Abs to Protein Kinases

18027298

P31749

55,686 Da

AKT Phosphorylates Targets In The Cytosol Pathway (106475); AKT Phosphorylates Targets In The Nucleus Pathway (106476); AKT-mediated Inactivation Of FOXO1A Pathway (106333); Activation Of BAD And Translocation To Mitochondria Pathway (105659); Activation Of BH3-only Proteins Pathway (105658); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adaptive Immunity Signaling Pathway (366160); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505)

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Akt1: an oncogenic AGC kinase that plays a critical role in regulating cell survival and metabolism in many different signaling pathways. Dual phosphorylation is required for its activation. T308 is phosphorylated by PDK1 in the PI3 kinase pathway, and S473 is phosphorylated by mTOR in the mTORC2 pathway. The Lys-63 -linked ubiquitination of AKT1 by TRAF6 is important for its translocation to the plasma membrane, phosphorylation, and activation. When Akt is fully phosphorylated it translocates into the nucleus, undergoes Lys-48 -polyubiquitination catalyzed by TTC3, leading to its proteosomal degradation. Hyperactive or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Over 160 protein substrates are known including many that regulate transcription, metabolism, apoptosis, cell cycle, and growth. Protein type: Oncoprotein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; EC 2.7.11.1; Kinase, protein; AGC group; AKT family. Chromosomal Location of Human Ortholog: 14q32.32. Cellular Component: microtubule cytoskeleton; nucleoplasm; mitochondrion; cytoplasm; plasma membrane; spindle; intercellular junction; nucleus; cytosol. Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; enzyme binding; phosphatidylinositol-3,4,5-triphosphate binding; protein kinase C binding; nitric-oxide synthase regulator activity; protein serine/threonine/tyrosine kinase activity; kinase activity; phosphatidylinositol-3,4-bisphosphate binding; ATP binding; protein kinase activity. Biological Process: negative regulation of JNK cascade; regulation of myelination; positive regulation of nitric oxide biosynthetic process; nerve growth factor receptor signaling pathway; protein ubiquitination; glucose homeostasis; regulation of cell migration; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; protein amino acid phosphorylation; germ cell development; positive regulation of glucose import; cell projection organization and biogenesis; protein catabolic process; maternal placenta development; response to food; platelet activation; glycogen biosynthetic process; fibroblast growth factor receptor signaling pathway; positive regulation of nitric-oxide synthase activity; positive regulation of blood vessel endothelial cell migration; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of cell growth; insulin-like growth factor receptor signaling pathway; cellular response to insulin stimulus; response to heat; T cell costimulation; negative regulation of protein kinase activity; striated muscle cell differentiation; positive regulation of fat cell differentiation; positive regulation of transcription factor activity; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of nitric-oxide synthase activity; response to oxidative stress; negative regulation of apoptosis; negative regulation of autophagy; negative regulation of fatty acid beta-oxidation; translation; apoptosis; protein amino acid autophosphorylation; positive regulation of glycogen biosynthetic process; regulation of glycogen biosynthetic process; positive regulation of cellular protein metabolic process; negative regulation of cell size; negative regulation of caspase activity; glucose transport; signal transduction; nitric oxide metabolic process; regulation of translation; apoptotic mitochondrial changes; protein kinase B signaling cascade; nitric oxide biosynthetic process; cell differentiation; inflammatory response; activated T cell apoptosis; aging; epidermal growth factor receptor signaling pathway; negative regulation of proteolysis; phosphoinositide-mediated signaling; myelin maintenance in the peripheral nervous system; protein modification process; positive regulation of peptidyl-serine phosphorylation; endocrine pancreas development; osteoblast differentiation; cell proliferation; G-protein coupled receptor protein signaling pathway; peptidyl-serine phosphorylation; protein import into nucleus, translocation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; insulin receptor signaling pathway; positive regulation of vasoconstriction; innate immune response; gene expression; positive regulation of protein amino acid phosphorylation; blood coagulation; vascular endothelial growth factor receptor signaling pathway; hyaluronan metabolic process; phosphorylation. Disease: Schizophrenia; Cowden Syndrome 6; Proteus Syndrome; Breast Cancer; Ovarian Cancer

0.1 mg

570 USD