catalog number :
MBS601701
products full name :
Fas, Neutralizing (CD95, APO-1)
products short name :
Fas, Neutralizing
products name syn :
Anti -Fas, Neutralizing (CD95, APO-1)
specificity :
Recognizes the human cell surface antigen Fas expressed in various human cells, including myeloid cells, T lympho-blastoid cells and diploid fibroblasts. Does not induce apoptosis in cell culture. Species Crossreactivity: Does not react with TNF and mouse.
purity :
Affinity Purified. Purified by ammonium sulfate precipitation and Protein A affinity chromatography.
form :
Supplied as a liquid in PBS, 50% glycerol.
storage stability :
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
tested application :
Western Blot (WB), Flow Cytometry (FC/FACS), Neutralization
app notes :
Suitable for use in Neutralization, Flow Cytometry and Western Blot. Dilution: Neutralization: 500ng/ml neutralized over 50% of the apoptosis induced by F0019-64 Human Fas, Activating, treatment of human Jurkat cells. Western Blot: 5ug/ml. Flow Cytometry: 5-20ug/ml
other info1 :
Immunogen: Recombinant human Fas.
products categories :
Antibodies; Abs to Apoptosis
products description :
Apoptosis or programmed-cell death is a physiological process essential for the normal development and maintenance of homeostasis in many organisms. This "cellular suicide" can be mediated by the Fas antigen (CD95, APO1). Fas antigen is a cell-surface glycoprotein (40-50kD). It belongs to the nerve growth factor/tumor necrosis factor (TNF) receptor family. Mice carrying the lymphoproliferation (lpr) mutation develop lymphoadenopathy and suffer from a systematic lupus erythematosus-like autoimmune disease. This mutation is a defect in the Fas gene. Fas is expressed in several tissues including blood, where it expression is upregulated on activated T and B lymphocytes and natural killer cells.