catalog number :
MBS601678
products full name :
Mycobacterium tuberculosis, Early Secreted Antigenic Target Protein (ESAT6)
products short name :
Mycobacterium tuberculosis, Early Secreted Antigenic Target Protein
products name syn :
Anti -Mycobacterium tuberculosis, Early Secreted Antigenic Target Protein (ESAT6)
specificity :
Recognizes a 10kD band from short term culture of M. tuberculosis identified as the protein ESAT-6 (Rv3875) in Western Blot. Binds to an epitope consisting of the residues EQQWNFAGIEAAA at positions 3-15 (4). Species Crossreactivity: Mycobacterium kansasii, Mycobacterium szulgai, and Mycobacterium marinum. Reacts with PPD, culture filtrate, cytosol and cell wall preparations from M. tuberculosis.
purity :
Affinity Purified. Purified by Protein A affinity chromatography from culture supernatant.
form :
Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide before the addition of glycerol to 40%. No stabilizing proteins added.
concentration :
0.05mg/ml
storage stability :
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
tested application :
ELISA (EL/EIA), Western Blot (WB)
app notes :
Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:3160. Optimal dilutions. to be determined by the researcher.
other info1 :
Immunogen: PPD from Mycobacterium tuberculosis (Tuberculin PPD, RT23, Statens Serum Institut) (1). Culture Medium: RPMI 1640 with 10% fetal calf serum.
other info2 :
Hybridoma: X63-Ag8.653. Female CF1 x Balb/c mice immunized i.p.
products categories :
Antibodies; Abs to Infectious Disease Mycobacterium
products description :
The ESAT-6 antigen from Mycobacterium tuberculosis is a dominant target for cell-mediated immunity in the early phase of tuberculosis (TB) in TB patients as well as in various animal models (2,5).