antibody

Reovirus Type 3

MBS601350

0.1 mg

495 USD

monoclonal

mouse

nonconjugated

[2Q2048]

Antibody

Reovirus Type 3

[Reovirus Type 3]

[Anti-Reovirus Type 3]

Monoclonal

IgG2a

[2Q2048]

Mouse

Human

Recognizes the hemaglutinin protein of Reovirus Type 3. Specific for the sigma 1 hemagglutinin protein of Type 3 Reovirus. Neutralizes reovirus type 3 and inhibits the hemagglutinating activity of the virus.

Purified by Protein G affinity chromatography.

Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Neutralization

Suitable for use in Neutralizaton and Hemagglutination Inhibition. Other applications not tested. Recommended Dilutions: Hemagglutination Inhibition: 10-20ug/ml. Dilute with buffer pH 7.5-8.0 to desired working volumes. For extensive dilution, protein-containing or other stabilizing medium should be used. Incubate diluted and virons (4HA units, 1x10e11 particles) in small volumes (25ul) at 37 degree C for 1 hour in 96 well round bottom plates. Prepare human type A erthrocytes (wash and suspend at a concentration of 2% v/v) and add 50ul to the virion-antibody mixtures. Incubate overnight at 4 degree C. The HA inhibition (HAI) titer is defined as the lowest concentration of antibody capable of inhibiting HA (typically > 3ug/ml). Optimal dilutions to be determined by the researcher

Immunogen: Purified reovirus type 3 virons

Antibodies; Abs to Infectious Disease

Reovirus type 3 (Reo-3) can infect many different rodent species and is prevalent in laboratory rodent colonies. It is a common contaminant of transplantable tumor cell lines and other biological materials. Infection of neonatal mice with Reo-3 can lead to a multi-systematic disease that is characterized by necrotizing hepatitis, myocarditis, pancreatitis, and meningoencephalitis. Steatorrhoea often develops secondary to liver disease and results in "oily skin" appearance.

1. Burstin, S.J., et al., Virology 117(1): 146-155 (1982). 2. William, W.V., et al., PNAS USA 85(17): 6488-6492 (1988). 3. Noseworthy, J.H., et al., J. Immunology 131(5): 2533-2538 (1983). 4. Chappell, J.D., et al., J. Virology 74(18): 8472-8479 (2000). 5. Barton, E.S., et al., J. Biol. Chem. 276(3): 2200-2211 (2001). 6. Nason, E.L., et al., J. Immunology 75(14): 6625-6634 (2001). 7. Monfardini, C., et al., J. Biol. Chem. 273(13): 7657-7667 (1998).

0.1 mg

495 USD