antibody

Parvovirus B19, Human

MBS601255

0.1 mg

485 USD

monoclonal

mouse

nonconjugated

[2Q2041]

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

Antibody

Parvovirus B19, Human

[Parvovirus B19]

[Anti -Parvovirus B19, Human]

Monoclonal

IgG1

[2Q2041]

Mouse

Human

Recognizes human Parvovirus B19. Recognizes an epitope common to VP1 and VP2 structural proteins of human Parvovirus B19. The epitope has been mapped to aa328-344 of VP2 capsid protein. Staining is localized predominantly in the nucleus though the cytoplasmic staining may be observed.

Purified by Protein A affinity chromatography from ascites.

Supplied as a liquid in 0.2M PBS, pH 7.6, 0.1% sodium azide.

1mg/ml

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Western Blot. Dilution: ELISA: 1:200. Immunofluorescence: 1:200-1:400. Immunohistochemistry (Paraffin): 1:50-1:100. High temperature citrate buffer antigen retrieval is highly recommended. Western Blot: 1:1000. Optimal dilutions to be determined by the researcher.

Immunogen: Native parvovirus B19 purified from human plasma. Grade: Affinity Purified

Positive Control: Parvovirus positive patient sample

Antibodies; Abs to Infectious Disease

Parvovirus B19 infection is common among children and adults and is often asymptomatic. Parvovirus B19 infection can result in serious and occasionally fatal hematologic diseases in susceptible patients, and is the cause of "fifth disease", a highly contagious infection of childhood. In sickle cell disease and other hereditary and acquired hemolytic anemias, acute B19 infection results in transient aplastic crisis, in which hemoglobin levels precipitously fall, anemia becomes life threatening, and congestive heart failure may ensue. Parvovirus B19 can also infect the fetus causing severe anemia, congestive heart failure, and heath in utero or at birth.

0.1 mg

485 USD