antibody

PERK, phosphorylated (Thr980) (PKR-like ER Resident Kinase)

MBS601178

0.1 mL

455 USD

monoclonal

rabbit

nonconjugated

7H50

western blot, ELISA, enzyme immunoassay

Antibody

PERK, phosphorylated (Thr980) (PKR-like ER Resident Kinase)

PERK

Anti -PERK, phosphorylated (Thr980) (PKR-like ER Resident Kinase)

pancreatic eIF-2alpha kinase, isoform C; Eukaryotic translation initiation factor 2-alpha kinase; CG2087 gene product from transcript CG2087-RA; CG2087-PA; CG2087-PB; CG2087-PC; PEK-PA; PEK-PB; PEK-PC; PERK; pancreatic eIF-2alpha kinase; pancreatic eIF2alpha kinase; pancreatic eIF-2alpha kinase; PRKR-like endoplasmic reticulum kinase

PEK; PEK; CG2087; DmelCG2087; DmPEK; DpERK; DPERK; EIF2-like; perk; pERK; Perk; PERK; EIF2AK3; DmPEK; PEK; PERK

E2AK3_DROME

Monoclonal

IgG

7H50

Rabbit

Rat

Recognizes endogenous levels of PERK phosphorylated at Thr980 at ~170kD. Species Crossreactivity: rat.

Supernatant. Supernatant

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

ELISA (EL/EIA), Western Blot (WB)

Suitable for use in ELISA and Western Blot. Dilution: Western Blot: 1:1000

Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Thr980 of mouse PERK, conjugated to KLH. Species Sequence Homology: mouse.

Antibodies; Abs to Protein Kinases

PERK, an eIF2 alpha kinase, is a transmembrane protein resident in the endoplasmic reticulum (ER) membrane and couples ER stress signals to translation inhibition. ER stress increases the activity of PERK, which then phosphorylates eIF2 alpha, resulting in reduced translation. PERK-deficient mice have defects in pancreatic beta cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress. PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain. Phosphorylation of PERK at Thr980 can serve as a marker for its activation status.

442617553

NP_001262283.1

NM_001275354.1

Q9NIV1

131,039 Da

Metabolism Of Proteins Pathway (824151); PERK Regulated Gene Expression Pathway (824210); Protein Processing In Endoplasmic Reticulum Pathway (167200); Protein Processing In Endoplasmic Reticulum Pathway (167190); Translation Factors Pathway (198275); Unfolded Protein Response Pathway (824205)

Function: Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis . By similarity. Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Enzyme regulation: Perturbation in protein folding in the endoplasmic reticulum (ER) promotes reversible dissociation from HSPA5/BIP and oligomerization, resulting in transautophosphorylation and kinase activity induction . By similarity. Subunit structure: Forms dimers with HSPA5/BIP in resting cells. Oligomerizes in ER-stressed cells . By similarity. Subcellular location: Endoplasmic reticulum membrane; Single-pass type I membrane protein . By similarity. Induction: By ER stress. Domain: The lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5/BIP . By similarity. Post-translational modification: Autophosphorylated . By similarity.N-glycosylated . By similarity. Sequence similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.Contains 1 protein kinase domain.

0.1 mL

455 USD