Integrin alphaV, beta5 | MyBioSource MBS601117 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Integrin alphaV, beta5

catalog :

MBS601117

quantity :

0.1 mg

price :

680 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[2Q873]

reactivity :

human

application :

ELISA, immunocytochemistry, immunoprecipitation, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS601117

products type :

Antibody

products full name :

Integrin alphaV, beta5

products short name :

[Integrin alphaV, beta5]

products name syn :

[Anti -Integrin alphaV, beta5]

other names :

[integrin beta-5; Integrin beta-5; integrin beta-5; integrin, beta 5]

other gene names :

[ITGB5; ITGB5]

uniprot entry name :

ITB5_HUMAN

clonality :

Monoclonal

isotype :

IgG2a

clone :

[2Q873]

host :

Mouse

reactivity :

Human

specificity :

Recognizes human integrin alphaVbeta5. Does not recognize integrin alphaVbeta3 or alpha5beta1. Binds to the alphaVbeta5 complex. Has a Kd of 2.8 nM in Mn2+, 4.0 nM in Ca2+and 2.9 nM in buffer lacking cation. Thus, cations do not significantly affect the binding to alphaVbeta5. Does not block ligand binding to purified alphaVbeta5, nor does it block cell adhesion mediated through this receptor.

purity :

Purified

form :

Supplied as a liquid in PBS. No preservative added.

concentration :

1mg/ml

storage stability :

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

tested application :

ELISA (EL/EIA), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)

app notes :

Recommended Dilutions: . Flow Cytometry: MG-63 cells have been harvested, blocked for 20 minutes at 4°( in 20% normal human serum then washing in medium containing 1 00/0 FRS~ 5x 1 Oe5 cells per tube were subsequently incubated with primary antibody for 30 minutes on ice, washed with medium, and incubated with fluorescent-conjugated secondary antibody. Immunocytochemistry: 1 Smg/ml for immunostaining of cells adhering to vitronectin-coated coverslips and fixed in 1 % paraformaldehyde containing 0.5% Triton X-l 00 for 20 minutes. Incubation for 30 minutes at 22°(, followed by detection with a fluorescein-conjugated secondary antibody. Optimal dilutions to be determined by the researcher.

other info1 :

Immunogen: Generated against human integrin alphaVbeta5 purified from placental tissue and depleted of integrin alphaVbeta3.

products categories :

Antibodies; Abs to Integrin

products description :

Integrins are transmembrane proteins that mediate interactions between adhesion molecules on adjacent cells and/or the extracellular matrix (ECM). Integrins have diverse roles in several biological processes including cell migration during development and wound healing, cell differentiation, and apoptosis. Their activities can also regulate the metastatic and invasive potential of tumor cells. They exist as heterodimers consisting of alpha and beta subunits. Some alpha and beta subunits exhibit specificity for one another and may be designated as a VLA (very late antigen) member. Heterodimers often preferentially bind certain cell adhesion molecules, or constituents of the ECM. Although they have no catalytic activity, integrins can be part of multimolecular signaling complexes known as focal adhesions.

ncbi gi num :

20127446

ncbi acc num :

NP_002204.2

ncbi gb acc num :

NM_002213.3

uniprot acc num :

P18084

ncbi mol weight :

88,054 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Antigen Processing-Cross Presentation Pathway (477122); Arrhythmogenic Right Ventricular Cardiomyopathy Pathway (672454); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117293); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Cross-presentation Of Particulate Exogenous Antigens (phagosomes) Pathway (477123); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); ECM Proteoglycans Pathway (833812)

uniprot summary :

ITGB5: Integrin alpha-V/beta-5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand. Heterodimer of an alpha and a beta subunit. Beta-5 associates with alpha-V. Interacts with MYO10. Belongs to the integrin beta chain family. Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral. Chromosomal Location of Human Ortholog: 3q21.2. Cellular Component: focal adhesion; cell surface; leading edge; plasma membrane; phagocytic vesicle; receptor complex. Molecular Function: integrin binding; protein binding; receptor activity. Biological Process: integrin-mediated signaling pathway; antigen processing and presentation of peptide antigen via MHC class I; extracellular matrix organization and biogenesis; muscle contraction; transforming growth factor beta receptor signaling pathway; cell-matrix adhesion; stress fiber formation; antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent

size1 :

0.1 mg

price1 :

680 USD

more info or order :

MyBioSource product webpage