antibody

HLA Class 1 Antigen B8

MBS601017

0.1 mL

410 USD

monoclonal

mouse

nonconjugated

[4i108]

Antibody

HLA Class 1 Antigen B8

[HLA Class 1 Antigen B8]

[Anti -HLA Class 1 Antigen B8]

[HLA class I histocompatibility antigen, B-8 alpha chain; MHC class I antigen; major histocompatibility complex, class I, B; OTTHUMP00000029212; lymphocyte antigen; MHC class I antigen SHCHA; MHC Class I HLA heavy chain; MHC class I antigen GN00104; leukocyte antigen class I-B; MHC HLA-B cell surface glycoprotein; MHC HLA-B transmembrane glycoprotein; MHC class I antigen HLA-B heavy chain; HLA class I histocompatibility antigen, B alpha chain; major histocompatibility complex, class I, B]

[HLA-B; HLA-B; AS; HLAB; SPDA1; MGC111087]

O02861_HUMAN

Monoclonal

IgM

[4i108]

Mouse

Human

Recognizes HLA Class 1 Antigen-B8. Specificity was determined at a 1:10 dilution by the Microcytotoxicity test under standard NIH conditions.

Ascites. Ascites

Supplied as a lyophilized powder from PBS, 1% BSA. Reconstitute with 100ul sterile ddH2O to make a 10X stock solution.

Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

This antibody has not been tested in methodologies other than Microcytotoxicity Test. Potential applications include Flow Cytometry, Cell Typing, Tissue staining and Chimerism Studies.

Cytotoxicity: 1:10. Add 0.9ml of PBS, 1% BSA to 100ul of 10X working dilution. Optimal dilutions to be determined by the researcher.

Cytotoxicity: Expected Results:. Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction. Limitations:. Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.

Antibodies; Abs to HLA

231381

O02861

Adaptive Immunity Signaling Pathway (366160); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway (366163); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480)

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq]

Sequence similarities: Belongs to the MHC class I family. RuleBase RU004439

0.1 mL

410 USD