antibody

HLA Class 1 Antigen B17,B35,B44(X-Reactive)

MBS601015

0.1 mL

410 USD

monoclonal

mouse

nonconjugated

[8.L.230]

Antibody

HLA Class 1 Antigen B17,B35,B44(X-Reactive)

[HLA Class 1 Antigen B17,B35,B44(X-Reactive)]

[Anti -HLA Class 1 Antigen B17,B35,B44(X-Reactive)]

Monoclonal

IgM

[8.L.230]

Mouse

Human

Recognizes human HLA Class 1Antigen-B17,B35,B44. Additional cross reactivity occurs with related proteins. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.

Ascites

Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH2O to make a 10X stock solution.

Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Microcytotoxicity Test

This antibody has not been tested in methodologies other than Microcytotoxicity Test. Potential applications include Flow Cytometry, Cell Typing, Tissue Staining and Chimerism Studies.

Directions for Use: For a cytotoxic working dilution, add 0.9ml of 1% BSA in PBS to 100ul of10X working stock. Store aliquots at -20°C. Expected Values: Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction. Limitations: Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.

Antibodies; Abs to HLA

HLA CLass I is involved in the presentation of foreign antigens to the immune system. This antibody is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders.

0.1 mL

410 USD