Semaphorin 5A (Semaphorin-5A, Semaphorin F, Sema F, SEMA5A, SEMAF) | MyBioSource MBS6009835 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Semaphorin 5A (Semaphorin-5A, Semaphorin F, Sema F, SEMA5A, SEMAF)

catalog :

MBS6009835

quantity :

0.1 mg

price :

570 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human

more info or order :

MyBioSource product webpage

image

image 1 :

Staining of paraffin embedded human kidney using MBS6009835 (4ug/ml). Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

product information

catalog number :

MBS6009835

products type :

Antibody

products full name :

Semaphorin 5A (Semaphorin-5A, Semaphorin F, Sema F, SEMA5A, SEMAF)

products short name :

[Semaphorin 5A]

products name syn :

[Anti -Semaphorin 5A (Semaphorin-5A, Semaphorin F, Sema F, SEMA5A, SEMAF)]

other names :

[semaphorin-5A; Semaphorin-5A; semaphorin-5A; sema F; semaphorin F; semaphorin-F; sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A; Semaphorin-F]

other gene names :

[SEMA5A; SEMA5A; semF; SEMAF; SEMAF; Sema F]

uniprot entry name :

SEM5A_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Human

specificity :

Recognizes human Semaphorin 5A.

purity :

Affinity Purified. Purified by immunoaffinity chromatography.

form :

Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

concentration :

0.5 mg/ml

storage stability :

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

tested application :

Suitable for use in ELISA (EIA), Western Blot (WB), and Immunohistochemistry (IHC). Other applications not tested.

app notes :

ELISA 1:128,000. Western Blot 0.03 ug/ml. Immunohistochemistry (Paraffin) 4-6 ug/ml paraffin embedded human kidney shows membranous and vesicular staining in PCT cells. Optimal dilutions to be determined by the researcher.

image1 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen: Synthetic peptide corresponding to DEATKKACYSKGKS, from the internal region of human Semaphorin 5A. Species Sequence Homology: Bovine, canine, mouse and rat.

products categories :

Antibodies; Abs to Plexin, Semaphorin

products description :

Members of the semaphorin protein family are involved in axonal guidance during neural development. Semaphorin 5a is thought to have a bifunctional role in axon guidance, exerting both attractive and inhibitory effects on developing axons associated with limbic function. The thrombospondin repeats of Semaphorin 5a physically interact with both heparan sulfate proteoglycans and chondroitin sulfate proteoglycans, inducing attractive and inhibitory guidance cues, respectively. The biological responses to Semaphorin 5a are induced through binding to its high-affinity receptor, plexin B3, triggering intracellular signaling through Met.

ncbi gi num :

147904700

ncbi acc num :

NP_003957.1

ncbi gb acc num :

NM_003966.2

uniprot acc num :

Q13591

ncbi pathways :

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); Developmental Biology Pathway (477129); Other Semaphorin Interactions Pathway (119526); Semaphorin Interactions Pathway (119519)

ncbi summary :

This gene belongs to the semaphorin gene family that encodes membrane proteins containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene.[provided by RefSeq, Jan 2010]

uniprot summary :

SEMA5A: a single-pass type I membrane protein that may act as positive axonal guidance cues. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 5p15.2. Cellular Component: membrane; plasma membrane; integral to membrane. Molecular Function: heparan sulfate proteoglycan binding; syndecan binding; axon guidance receptor activity; semaphorin receptor binding. Biological Process: nervous system development; axon guidance; positive regulation of actin filament depolymerization; axonal fasciculation; negative regulation of cell adhesion; negative regulation of axon extension involved in axon guidance; diencephalon development; patterning of blood vessels; positive regulation of protein kinase B signaling cascade; blood vessel endothelial cell proliferation during sprouting angiogenesis; positive regulation of angiogenesis; cell-cell signaling; positive chemotaxis; positive regulation of axon extension involved in axon guidance; positive regulation of endothelial cell proliferation; cell adhesion

size1 :

0.1 mg

price1 :

570 USD

more info or order :

MyBioSource product webpage