antibody

Wnt3a (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member)

MBS600946

0.1 mL

465 USD

monoclonal

rabbit

nonconjugated

8A42

human, mouse

western blot, immunoprecipitation

Antibody

Wnt3a (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member)

Wnt3a

Anti -Wnt3a (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member)

WNT3A; Protein Wnt-3a; protein Wnt-3a; OTTHUMP00000035713; wingless-type MMTV integration site family, member 3A

WNT3A; WNT3A; MGC119418; MGC119419; MGC119420

WNT3A_HUMAN

Monoclonal

IgG

8A42

Rabbit

Mouse

Recognizes transfected Wnt3a protein in L cells and the conditioned medium from mouse L/Wnt3a cells at ~42kD. Species Crossreactivity: mouse. Species Sequence Homology human and rat, 100%

Supernatant. Supernatant

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB), Immunoprecipitation (IP)

Suitable for use in Western Blot and Immunoprecipitation. Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight. Immunoprecipitation: 1:50

Immunogen: Synthetic peptide corresponding to residues surrounding Leu256 of human Wnt3a.

Antibodies; Abs to Wnt Proteins

The Wnt family includes several secreted glycoproteins that play important roles in animal development. There are 19 Wnt genes in the human genome that encode functionally distinct Wnt proteins. Wnt members bind to the Frizzled family of seven-pass transmembrane proteins and activate several signaling pathway. The canonical Wnt/ beta-catenin pathway also requires a coreceptor from the low-density lipoprotein receptor family. Aberrant activation of Wnt signaling pathways is involved in several types of cancers.

14530679

P56704

39,365 Da

Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Canonical Wnt Signaling Pathway (138032); Class B/2 (Secretin Family Receptors) Pathway (106378); DNA Damage Response (only ATM Dependent) Pathway (198827); GPCR Ligand Binding Pathway (161020); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); Hedgehog Signaling Pathway (83063); Hedgehog Signaling Pathway (474)

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq]

WNT3A: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Interacts with PORCN. Interacts with APCDD1 and WLS. Component of the Wnt-Fzd-LRP5-LRP6 signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly in the complex with LRP6. Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. Belongs to the Wnt family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 1q42. Cellular Component: extracellular space; proteinaceous extracellular matrix; cell surface; Golgi lumen; endoplasmic reticulum lumen; early endosome membrane; plasma membrane; extracellular region. Molecular Function: protein domain specific binding; protein binding; frizzled binding; transcription coactivator activity; receptor agonist activity; frizzled-2 binding. Biological Process: positive regulation of mesodermal cell fate specification; axon guidance; extracellular matrix organization and biogenesis; positive regulation of protein binding; positive regulation of transcription, DNA-dependent; cell proliferation in forebrain; positive regulation of receptor internalization; positive regulation of caspase activity; Wnt receptor signaling pathway through beta-catenin; palate development; positive regulation of collateral sprouting in the absence of injury; negative regulation of axon extension involved in axon guidance; negative regulation of neurogenesis; Wnt receptor signaling pathway in forebrain neuroblast division; neuron differentiation; mammary gland development; positive regulation of cell proliferation; positive regulation of B cell proliferation; hemopoiesis; heart looping; dorsoventral neural tube patterning; inner ear morphogenesis; in utero embryonic development; hippocampus development; negative regulation of fat cell differentiation; positive regulation of peptidyl-serine phosphorylation; osteoblast differentiation; paraxial mesodermal cell fate commitment; signalosome assembly; spinal cord association neuron differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; positive regulation of protein amino acid phosphorylation

0.1 mL

465 USD