Axin2 | MyBioSource MBS600927 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Axin2

catalog :

MBS600927

quantity :

0.1 mL

price :

465 USD

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

7H7

reactivity :

human

application :

western blot, ELISA, immunoprecipitation, enzyme immunoassay

product information

catalog number :

MBS600927

products type :

Antibody

products full name :

Axin2

products short name :

Axin2

products name syn :

Anti -Axin2

other names :

AXIN2; Putative uncharacterized protein DKFZp781B0869; axin-2; conductin; axin-like protein; axis inhibition protein 2; axin 2

other gene names :

AXIN2; DKFZp781B0869; AXIL; MGC10366; MGC126582; DKFZp781B0869

uniprot entry name :

Q5H9R8_HUMAN

clonality :

Monoclonal

isotype :

IgG

clone :

7H7

host :

Rabbit

reactivity :

Human

specificity :

Recognizes endogenous levels of total human Axin2 protein at ~95, 98kD.

purity :

Supernatant. Supernatant

form :

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0. 02% sodium azide, 50% glycerol.

storage stability :

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

tested application :

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)

app notes :

Suitable for use in ELISA, Western Blot and Immunoprecipitation. Dilution: Western Blot: 1:1000. Immunoprecipitation: 1:50

other info1 :

Immunogen: Synthetic peptide corresponding to residues surrounding Pro566 of human Axin2 (KLH).

products categories :

Antibodies; Abs to Wnt Proteins

products description :

Axin1 (Axis inhibition protein 1) and Axin2 are multidomain scaffold proteins that negatively regulate Wnt signaling. Axin1 complexes with APC, GSK3 beta, Dvl and beta-catenin and promotes the GSK3 beta-mediated phosphorylation and subsequent degradation of beta-catenin. Upon stimulation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling. In addition to its role in the Wnt signaling pathway, Axin1 complexes with MEKK1 and causes the activation of c-Jun amino-terminal kinase (JNK/SAPK). Axin2 (also known as Conductin or Axil) can functionally substitute for Axin1 in mice. Axin2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop.

ncbi gi num :

6653584

uniprot acc num :

Q5H9R8

ncbi mol weight :

93,542 Da

ncbi pathways :

Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Canonical Wnt Signaling Pathway (138032); Colorectal Cancer Pathway (83106); Colorectal Cancer Pathway (518); Endometrial Cancer Pathway (83109); Endometrial Cancer Pathway (521); Pathways In Cancer (83105); Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway (169352); TGF-beta Receptor Signaling Pathway (198774)

ncbi summary :

The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. [provided by RefSeq]

uniprot summary :

axin 2: is a negative regulator of the Wnt pathway, which is critical in stem cell signaling, morphogenesis, the mesenchymal-epithelial transition, and many cancers. Axin functions as a tumor suppressor. Probably facilitates the phosphorylation of beta-catenin and APC by GSK3B, leading to their ubiquitination and subsequent proteolysis. Is downregulated during progression of esophageal squamous cell carcinoma. Axin1/2 mediate cross-talk between TGF-beta and Wnt signaling pathways. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 17q24.1. Cellular Component: Golgi apparatus; centrosome; postsynaptic density; cytoplasmic membrane-bound vesicle; cell cortex; beta-catenin destruction complex; cytosol; microtubule cytoskeleton; nucleoplasm; cytoplasmic microtubule; cytoplasm; plasma membrane; nucleus. Molecular Function: protein binding; enzyme binding; ubiquitin protein ligase binding; beta-catenin binding; protein kinase binding; GTPase activator activity. Biological Process: odontogenesis; negative regulation of cell proliferation; cell proliferation; intramembranous ossification; regulation of mismatch repair; mRNA stabilization; dorsal/ventral axis specification; negative regulation of osteoblast differentiation; positive regulation of protein amino acid phosphorylation; maintenance of DNA repeat elements; bone mineralization; positive regulation of GTPase activity. Disease: Oligodontia-colorectal Cancer Syndrome

size1 :

0.1 mL

price1 :

465 USD