catalog number :
MBS600861
products full name :
HLA Class 1 Antigen A1,A11,A26 (X-Reactive)
products short name :
[HLA Class 1 Antigen A1,A11,A26]
products name syn :
[Anti -HLA Class 1 Antigen A1,A11,A26 (X-Reactive)]
specificity :
Reacts to HLA Class 1 Antigen-A1,A11,A26. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.
form :
Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH2O to make a 10X stock solution.
storage stability :
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Reconstitute with sterile ddH20. Aliquot to avoid repeated freezing and thawing. Store at -20°C . For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
tested application :
This antibody has not been tested in methodologies other than Microcytotoxicity Test. Potential applications include Flow Cytometry, Cell Typing, Tissue Staining and Chimerism Studies.
app notes :
Recommended Dilutions: . Cytotoxicity: Add 0.9ml of 1% BSA in PBS to 100ul of 10X working dilution. Optimal dilutions to be determined by the researcher.
other info1 :
Immunogen: Human cells expressing A1, A11 and A26 antigens. Cytotoxicity: Expected Results:. Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction. Limitations:. Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.
products categories :
Antibodies; Abs to HLA